Thermo Scientific Dionex TCC-100 Operating Instructions Manual
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Thermo Scientific Dionex TCC-100 Operating Instructions Manual

Thermostatted hplc column compartment
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Thermostatted HPLC
Column Compartment
Operating Instructions
Revision:
Date:
TCC-100
3.00
April, 2004
©
2004 Dionex
Doc.: TCC100_OI_E_V3_0.doc

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Summary of Contents for Thermo Scientific Dionex TCC-100

  • Page 1 TCC-100 Thermostatted HPLC Column Compartment Operating Instructions Revision: 3.00 Date: April, 2004 © 2004 Dionex Doc.: TCC100_OI_E_V3_0.doc...
  • Page 2 TCC-100 Operating Instructions...
  • Page 3 Declaration of Conformity Product: Thermostatted HPLC Column Compartment Type: TCC-100 Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations: • Low-Voltage Equipment Directive 73/23/EEC changed by 93/68/EEC • EMC Directive 89/336/EEC changed by 91/263/EEC; 92/31/EEC; 93/68/EEC The electrical safety of the products was evaluated based on the following standard: •...

  • Page 5: Table Of Contents

    TCC-100 Operating Instructions Table of Contents Introduction ........................1 How to Use This Manual ................... 1 Unpacking ........................2 Intended Use....................... 3 Federal Communications Commission (FCC) Note ..........3 Overview..........................5 Product Overview....................... 5 Supported Configurations ..................5 Theory of Operation ....................6 Column Switching Valve ...................
  • Page 6 TCC-100 Operating Instructions Maintenance ........................49 General Notes......................49 Periodical Maintenance .................... 49 Replacing the Fuses....................50 Technical Information..................... 51 Accessories and Spare Parts ................... 53 Standard Accessories (included in the shipment) ............ 53 Optional Accessories....................53 Spare Parts........................ 54 Technical Appendix - Column Switching Valve............

  • Page 7: Introduction

    TCC-100 Operating Instructions 1 Introduction 1.1 How to Use This Manual The layout of this manual is designed to provide quick reference to the sections of interest to the user. However, in order to fully understand the thermostatted column compartment, we recommend that you review the manual thoroughly before beginning operation of the column compartment.

  • Page 8: Unpacking

    TCC-100 Operating Instructions 1.2 Unpacking All electrical and mechanical components of the column compartment are carefully tested before the instrument is shipped from the factory. After unpacking, please inspect the compartment for any signs of mechanical damage that may have occurred during transit. Please note: Immediately report any shipping damage to both the incoming carrier and Dionex.

  • Page 9: Intended Use

    TCC-100 Operating Instructions 1.3 Intended Use The column compartment is designed to perform equally well as a dependable system for routine analyses or as a sophisticated research instrument, especially for HPLC (high performance liquid chromatography) applications. The column compartment can be operated manually, from the front panel, or controlled remotely by the Chromeleon Chromatography Management System.
  • Page 10 TCC-100 Operating Instructions...

  • Page 11: Overview

    TCC-100 Operating Instructions 2 Overview 2.1 Product Overview The column compartment is designed to perform equally well as a dependable system for routine analyses or as a sophisticated research instrument, especially for HPLC applications. The temperature can be set on the instrument itself, using the front panel controls or remotely, via Chromeleon.

  • Page 12: Theory Of Operation

    TCC-100 Operating Instructions For an overview of the currently available configurations, refer to the table below. If you have any questions, please contact the Dionex Sales department or your Dionex distributor. Model Part No. Description TCC-100 5710.0000 Thermostatted column compartment (basic version) TCC-100 MSV 2P-6P 5710.0010 Thermostatted column compartment with...

  • Page 13: Column Switching Valve

    TCC-100 Operating Instructions 2.4 Column Switching Valve For applications that require two different columns at frequent intervals at similar temperatures, Dionex also offers the TCC-100 Thermostatted Column Compartment with a 2-position/6-port or 2-position/10-port column switching valve (part numbers: 5710.0010 for the TCC-100 with 2-position/6-port valve;...

  • Page 14: Front Panel Display And Controls

    TCC-100 Operating Instructions 2.5 Front Panel Display and Controls Fig. 3: Front panel Front Panel Function Element Switches the instrument to stand-by mode. Increases the set point temperature in increments of 0.1°C (→ Operation, page 23) Sets the leak detection mode (→ Setting the Leak Detection Mode, page 23) Decreases the set point temperature in increments of 0.1°C (see above) Sets the leak detection mode (see above) Temperature...

  • Page 15: Safety Precautions

    TCC-100 Operating Instructions 2.6 Safety Precautions Please observe the following general safety precautions while operating the instrument or carrying out any maintenance work: Important: When lifting or moving the column compartment, lift only from the bottom or sides of the instrument. Lifting the column compartment by the front panel may damage the hinges of the front panel door.
  • Page 16 TCC-100 Operating Instructions...

  • Page 17: Installation

    TCC-100 Operating Instructions 3 Installation Bring the unit to a moderate temperature for four hours to allow any condensation that might have occurred during shipping to evaporate. Do not connect the column compartment to the mains yet. After four hours, check the instrument; if the condensation is still there, allow the instrument to continue to warm up (without connecting it to the mains) until the condensation is completely gone.

  • Page 18: Electrical Connection

    TCC-100 Operating Instructions 3.2 Electrical Connection Use the power cable provided in the accessories kit to connect the column compartment to the mains. The column compartment is equipped with a standard power unit with an automatic voltage selector. Thus, no adjustment is required to adapt the line voltage to the local voltage requirements.

  • Page 19: Usb

    TCC-100 Operating Instructions 3.3.1 USB Connect the column compartment to the server PC via the USB port (→ Fig. 5, no. 7). To do so, select one of the following alternatives: • Connect the column compartment directly to the USB port on the server PC. •...

  • Page 20: Opening The Front Panel

    TCC-100 Operating Instructions 3.4 Opening the Front Panel The column compartment front panel tilts downward to provide easy access to the columns and capillaries installed in the column chamber. To open the door, press the release button on top of the housing.

  • Page 21: Installing A Column

    TCC-100 Operating Instructions 3.5 Installing a Column • Press the column(s) into the column clip(s) (→ Fig. 9): Fig. 9: Column in column clip • Connect the capillaries. Please note: For information about how to connect the capillaries if a column switching valve is used, refer to section 3.7 (→...

  • Page 22: Installing The Eluent Preconditioner

    TCC-100 Operating Instructions 3.6 Installing the Eluent Preconditioner The eluent preconditioner brings the solvent to the column temperature before the solvent enters the column. This avoids temperature gradients on the column. Two eluent preconditioners are available from Dionex as an option for the column compartment: Fig.

  • Page 23: Installing The Eluent Preconditioner Without Column Clips

    TCC-100 Operating Instructions 3.6.1 Installing the Eluent Preconditioner without Column Clips (Unlike on the photos, the color of the preconditioner is black (→ Fig. 11 ). • Tighten the bottom parts of the preconditioner holders at the desired position in the column chamber.
  • Page 24 TCC-100 Operating Instructions • Installation of the eluent preconditioner is now complete. Fig. 15: Installed eluent preconditioner • Install the column at an appropriate position in the column chamber. Fig. 16: Column and eluent preconditioner installed • Connect the capillary from the autosampler or pump to the preconditioner inlet, connect the preconditioner outlet to the column and then, connect the column outlet to the detector.

  • Page 25: Installing The Eluent Preconditioner With Column Clips

    TCC-100 Operating Instructions 3.6.2 Installing the Eluent Preconditioner with Column Clips (Unlike on the photos, the color of the preconditioner is black (→ Fig. 11 ). • Tighten the bottom parts of the preconditioner holders at the desired position in the column chamber and push the preconditioner into the holders as shown below.
  • Page 26 TCC-100 Operating Instructions • Tighten the top parts of the preconditioner holders into place (→ Fig. 14, page 17). • Install the column clips as shows in Fig. 21. Fig. 21: Installing the column clips • Installation of the eluent preconditioner is now complete. Fig.

  • Page 27: Connecting The Capillaries To The Column Switching Valve

    TCC-100 Operating Instructions 3.7 Connecting the Capillaries to the Column Switching Valve If the column compartment is fitted with a column switching valve, valve switching is programmed and controlled via Chromeleon. (For more information, refer to the Chromeleon online Help.) Column identification system Column switching valve Fig.

  • Page 28: Leak Sensors

    TCC-100 Operating Instructions 3.8 Leak Sensors Please note: Inspect the column compartment for leaks every day. Tighten leaking capillary connections. The column compartment contains two leak sensors to detect any humidity or gases that may accumulate in the column chamber. If one of the sensors detects a leak, an acoustic beep sounds. Humidity and gases in the column chamber will be detected by the humidity or gas sensor, respectively.

  • Page 29: Stand-Alone Control

    TCC-100 Operating Instructions 4 Stand-alone Control Please observe the information about the facility requirements (→ page 11) and the electrical connection (→ page 12). Use the power cord shipped with the column compartment to connect the instrument to the mains. You do not need to take any further action. 4.1 Operation Turn on the column compartment by pressing the power switch on the rear panel.

  • Page 30: Operation After A Power Failure

    TCC-100 Operating Instructions After you have entered the desired value, wait for about 7 seconds until the display stops flashing. Do not press any other key during this time. The column compartment then stores the settings and displays the actual temperature value again. Please note: If the sensitivity has been set in Chromeleon (→...

  • Page 31: Automated Control By Chromeleon

    TCC-100 Operating Instructions 5 Automated Control by Chromeleon 5.1 General If desired, the column compartment can be controlled by the Chromeleon Chromatography Management System. To control the column compartment by the data system, make sure that the following license is available under Chromeleon. (If you have any questions, please contact your Dionex Sales Representative.) •...

  • Page 32: Lan Installation

    TCC-100 Operating Instructions 5.1.2 LAN Installation Please note: Install the LAN connection under Windows NT 4.0 only. For any other operating systems, we recommend installing a USB connection. Connect the column compartment to the mains using the power cord shipped with the instrument.
  • Page 33 TCC-100 Operating Instructions If you wish to connect several TCP/IP-enabled Summit HPLC devices to the server PC, you must use a hub (10 Mbit) to establish the connection to the instrument LAN. In this case, use standard network cables instead of the crossover cable (One standard network cable, part no. 8906.2038, is provided in the accessories kit.) Fig.
  • Page 34 TCC-100 Operating Instructions Configure the network adapter for the instrument LAN (here: [2] 3Com Fast EtherLink XL NIC (3C905B-TX)) on the Bindings tab page. First, select all protocols in the Show Bindings for field to verify that the TCP/IP protocol is installed. Install the TCP/IP protocol, if necessary: Fig.
  • Page 35 TCC-100 Operating Instructions An IP address and a subnet mask are required for the separate network adapter because the TCP/IP protocol is used for the instrument LAN. The dialog box for entering the IP address and the subnet mask is opened automatically during installation. Please note: You can reopen this dialog box later if you need to change the IP address and the subnet mask.
  • Page 36 TCC-100 Operating Instructions Note: The subnet mask determines which part of the IP address describes the network and which part defines the individual devices connected to the network. Independent LANs have different network addresses. Based on the subnet mask 255.255.255.0 in the above example, the term "192.168.60" describes the network, while "xxx"...

  • Page 37: Assigning Ip Properties To The Column Compartment (Cmiputil)

    TCC-100 Operating Instructions Example for a valid configuration for case B: Column compartment: IP address 192.168.60.51 Subnet mask 255.255.255.0 Server: IP address 192.168.60.50 Subnet mask 255.255.255.0 Important: Every device on the same network must have a unique IP address. Selecting duplicate IP addresses may cause conflicts on the network. Important: Do not use 0 or 255 as the last numbers in IP addresses.
  • Page 38 TCC-100 Operating Instructions • If necessary, copy the CmIPUtil program from the software CD to a separate directory on your computer. • Double-click the Setup.exe file to start installation. Follow the installation instructions as they appear on the screen. • Open the program via Start → Programs → Chromeleon → IP Utility → CmIPUtil. Fig.
  • Page 39 TCC-100 Operating Instructions Example for a valid configuration: Column compartment: IP address 192.168.60.51 Subnet mask 255.255.255.0 Server: IP address 192.168.60.50 Subnet mask 255.255.255.0 To configure several TCP/IP-enabled Summit HPLC devices, make sure that every device has its own IP address. In this example, the following entries would be possible: 2.

  • Page 40: Recommendations For Network Operation

    TCC-100 Operating Instructions 5.1.2.2 Recommendations for Network Operation Unlike USB connections, Ethernet LAN connections have limited real-time capabilities. Data transmission can occur only when no other connected user is using the network. In order to take full advantage of the real-time capabilities of the column compartment, select the instrument LAN connection (→...

  • Page 41: Installing The Column Compartment In Chromeleon

    • Select Dionex from the left list box, and then select TCC-100 Column Thermostat from the right list box. (For Chromeleon versions earlier than 6.50, select Dionex TCC-100 Column Thermostat from the drivers list.) Click OK to confirm your selection.
  • Page 42 TCC-100 Operating Instructions General Tab Page Define the general instrument parameters: Fig. 34: General tab page • Verify that Demo Mode is disabled. (If Demo Mode is enabled, the Module address section and the Firmware Download button are disabled.) • The Device Name field indicates the name under which the column compartment is identified in the installation environment.
  • Page 43 TCC-100 Operating Instructions The Address column lists all available modules. The entry that is available via Ethernet (TCP/IP) includes the module's IP address. The entry for a module that is available via USB (identified by the prefix "USB") includes the module's Ethernet address or serial number.
  • Page 44 TCC-100 Operating Instructions Configuration Tab Page Use the Configuration tab page to determine the configuration of the column compartment: Fig. 36: Configuration tab page Option Description Internal MSV Select this check box if the column compartment is equipped with a column switching valve.
  • Page 45 TCC-100 Operating Instructions Relays Tab Page The Relays tab page lists all available relays. (To access this tab page, select the column thermostat in the Server Configuration program, and then select Properties... on the context menu.) Click a check box to enable or disable the corresponding relay. Make sure that the required relays are selected;...

  • Page 46: Operation With Chromeleon

    TCC-100 Operating Instructions 5.3 Operation with Chromeleon Chromeleon can control the column compartment in two ways: • Directly, via the icon and menu commands or via the controls on the corresponding control panel. • Via time-based programs (PGM-File or programmable buttons). The following commands and properties are available;...
  • Page 47 TCC-100 Operating Instructions Commands/ Description Properties in Chromeleon Disconnect Disconnects the column compartment from Chromeleon (also see Connected, Connect). Door Indicates whether the compartment door is open or closed (Open or Closed; read-only). EquilibrationTime Range: 0.0…30.0 min. Only if the deviation between the current temperature and the set point specified under ReadyTempDelta does not exceed the EquilibrationTime, the column compartment enters the Ready state (also see ReadyTempDelta and Ready).
  • Page 48 TCC-100 Operating Instructions Commands/ Description Properties in Chromeleon Relay 2. Click the "+ "character beside the relay name to display the items TCC_RELAY_2 underneath: State−indicates the state of the relay. Duration−when set, the relay toggles after the specified time. On−turns the relay on. Off−turns the relay off.

  • Page 49: Column Identification System (Column Id)

    TCC-100 Operating Instructions LdA = In Chromeleon, a setting not allowed in stand-alone mode has been selected; for example, different sensitivity settings for the gas and humidity sensors. If the sensitivity is set in Chromeleon, this setting is remains valid when the column compartment operated in stand-alone mode after being disconnected from the data system.

  • Page 50: System Wellness And Reliability

    TCC-100 Operating Instructions Please note: To open the "Commands" dialog box, open a Chromeleon control panel and then, press the F8 key or select Command… on the Control menu. You can also open this dialog box by pressing the F8 key. In the left list box, click the "+"...

  • Page 51: Troubleshooting

    TCC-100 Operating Instructions 6 Troubleshooting 6.1 List of the Possible Problems The following table provides a summary of possible operating problems, lists probable causes, and suggests remedial actions: Problem Probable Cause Remedial Action No temperature display. The column compartment is not Connect the power cord.
  • Page 52 TCC-100 Operating Instructions Problem Probable Cause Remedial Action The system has very high The column is contaminated or Rinse or replace the column. backpressure. blocked. The capillaries are blocked or Remove the blockage or replace damaged by bending. the capillaries. The eluent preconditioner is Rinse both the capillary of the blocked.

  • Page 53: Chromeleon Error Messages

    TCC-100 Operating Instructions 6.2 Chromeleon Error Messages If communication between the data system and the column compartment cannot be established, an error message is displayed in the Chromeleon Audit Trail. The following table lists the most important error messages, along with possible causes and the corresponding remedial actions.
  • Page 54 TCC-100 Operating Instructions Message Probable Cause Remedial Action TCC100 - Host not found. Network problems: no connection to the Check the network settings for the column compartment. column compartment and network TCC100 - Permission denied. interface card. The network connection between the Chromeleon server and the TCC100 - Network is unreachable.

  • Page 55: Maintenance

    TCC-100 Operating Instructions 7 Maintenance 7.1 General Notes The column compartment is made of high-quality components and materials, which minimize maintenance requirements. The painted surfaces, as well as the display, are relatively resistant to weak acids, alkali, and organic solvents. Nevertheless, immediately wipe up all liquids spilled onto the column compartment's surface, using a lint-free cloth or paper.

  • Page 56: Replacing The Fuses

    TCC-100 Operating Instructions Please note: For information about maintenance procedures for the column switching valve, refer to Technical Appendix - Column Switching Valve (→ page 55). All other maintenance procedures must be performed by Dionex Service personnel. Have a Dionex Service Representative check the column compartment once a year to prevent contamination, excessive wear, etc.

  • Page 57: Technical Information

    TCC-100 Operating Instructions 8 Technical Information Environmental Range of use: Indoor use Conditions: Temperature: 10°C to 35°C (50°F to 95°F) Air humidity: 40 to 85% rel. humidity, non-condensing Overvoltage Category: II Polution degree: Operating Conditions:* Temperature range: +5°C to +85°C (+41°F to +185°F) When cooling: max.
  • Page 58 TCC-100 Operating Instructions...

  • Page 59: Accessories And Spare Parts

    TCC-100 Operating Instructions 9 Accessories and Spare Parts Accessories and spare parts for the column compartment are always maintained at the latest technical standard. Therefore, part numbers are subject to alteration. However, updated parts will always be compatible with the parts they replace. The part numbers always refer to the packing unit.

  • Page 60: Spare Parts

    TCC-100 Operating Instructions 9.3 Spare Parts Part No.* Description 6710.9001 TCC-100 Fuses kit including 10 2A fuses, idle, 5 x 20 mm 5 6.3A fuses, idle, 5 x 20 mm 754.C2-20R6 Rotor seal for 2-position/6-port switching valve 754.C2-10R0 Rotor seal for 2-position/10-port switching valve 754.C-2C06 Stator for 2-position/6-port switching valve 754.C-1C00...

  • Page 61: Technical Appendix - Column Switching Valve

    TCC-100 Operating Instructions 10 Technical Appendix - Column Switching Valve...
  • Page 62 TCC-100 Operating Instructions or rotor seal (part no.: see below) or stator (part no.: see below) (here: 2-position/6-port valve) Part No. Description 754.C2-20R6 Rotor seal for 2-position/6-port switching valve 754.C2-10R0 Rotor seal for 2-position/10-port switching valve 754.C-2C06 Stator for 2-position/6-port switching valve 754.C-1C00 Stator for 2-position/10-port switching valve...

  • Page 63: Index

    TCC-100 Operating Instructions 11 Index Accessories Inputs (Tab Dialog Box) ........39 Optional............53 Installation ............11 Standard ............53 Column............15 Automated Control..........25 Connections (Rear Panel).......12 Electrical Connection ........12 Eluent Preconditioner......16, 17, 19 LAN ...............26 Chromeleon Location ............11 Error Messages..........47 Network Interface Card (NIC) .......27 Installation Program ........35 USB..............25 Operation............40...
  • Page 64 TCC-100 Operating Instructions USB..............13 Theory of Operation ..........6 Relays (Tab Dialog Box) ........39 Troubleshooting Reliability............44 Chromeleon Error Messages ......47 Possible Problems ..........45 Troubleshooting:..........45 Safety Precautions..........9 Spare Parts ............53, 54 Specification ............51 Unit Description Stand-alone Control ..........23 Column Switching Valve .........7 Standard Accessories ..........53 Product Overview ..........5 System Wellness ..........44...

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