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2.2 Buffer Preparation
Buffer A
1. Empty the contents of the bottle labeled buffer A into a 500 ml
graduated cylinder and add filtered, high-quality water to 500 ml
volume.
2. Place the contents of the graduated cylinder into a 1 L side-arm flask
and drop in a stir bar. Cap the side arm flask, place it on a stir plate and
connect it to a vacuum source. Degas the buffer for approximately
15 minutes with gentle stirring.
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When degassing is complete, pour the buffer into a bottle and label
appropriately.
Buffer B
Prepare buffer B by following the same procedure for preparation of
buffer A.
2.3 Sample Preparation
1. Remove the aluminum cap from the anion exchange standards vial.
Slowly remove the rubber plug from the standards vial (the contents
may be under vacuum).
2. Add 1.0 ml of prepared buffer A to the vial.
3. Replace the rubber stopper and gently invert the vial to solubilize the
protein standards.
2.4 Installation of the UNO Q1 column
Remove end caps from the UNO Q1 column. Keeping tubing lengths
to a minimum, connect 1/16" tubing from port 4 of the inject valve to the
column inlet. Connect the column outlet to the bottom of the UV flow
cell. Secure the column in a vertical position.
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