BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 17

Section 3
Preparation for Use of the
BioLogic HR System
The procedures in this document are written for two configurations,
which are distinguished by the following.
• Manual: Uses a V7-3 Manual Inject Valve, a Model 2110 Fraction
Collector, and an SV3-2 Diverter Valve.
• Automated: Uses an AV7-3 Automatic Inject Valve and a Model
2128 Fraction Collector.
When you turn on the BioLogic HR System, the Manual Control
Screen is displayed (see Figure 6). This screen consists of a series of
boxes containing instrument faceplates which provide direct control of
the gradient pumps, a fraction collector, a chart recorder (apart from chart
speed, which is set on the recorder itself), the UV and conductivity detector
range settings and solenoid and automated valves.
1. Priming the BioLogic HR Gradient Pumps.
a. Immerse the gradient pump A and B inlet lines in a container of
b. Connect the syringe (supplied with the fittings kit) to the priming
c. Turn the priming port counter-clockwise to open the seal. Gently
d. Repeat this operation several times until no air bubbles are visible
e. Tighten the priming port by turning it clockwise.
f.
2. Moving the Inject Valve to Purge Position.
•
•
HPLC grade (filtered, degassed) or other high quality water.
port of pump head A.
withdraw the syringe plunger to draw water into the pump head.
in the inlet tubing.
Repeat this priming procedure for pump head B.
For a manual inject valve V7-3, move the arm to the Purge position
(left, then up, then left). The figure in the valve position window
displays P.
Flow from the gradient pumps will now go directly to waste,
bypassing any column or detector downstream of the valve.
To change the status of an automated inject valve AV7-3, go to the
Manual Screen displayed on the controller monitor.
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