BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 6

3. Purging the BioLogic Duo-Flow Pumps.
a. Make sure that the inject valve is in the Purge position.
b. Press the Purge buttons A and B on the front of the workstation.
The gradient pumps will run and the indicator lights will flash
green.
c. Run both pumps for 2 minutes and then press the purge buttons
again to stop the pump.
BioLogic Duo-Flow - Default - no method
File View Utilities Options
New Edit New
Method Method Run Browser
Gradient pump: F-10
Flow rate:
1.00 ml/min
Inlet A: 100 %
%
0
Inlet B:
High
psi
1000
limit:
Low
0 psi
limit:
Set
START STOP
SVT3-2 at port 1 SV5-4 at port 2
1
1 2
4
Gradient Pump: F-10
0.00 ml/min 0 %B 438 psi
Flowrate of the gradient pump
Fig. 1. Manual Control Screen.
4. Control of the BioLogic Duo-Flow Pumps.
The gradient pump parameters are set from the Manual Screen either by
clicking in the appropriate field and entering a value from the keyboard
or by using the spin arrows. You can set the flow rate between 0.1 to
10 ml/min and the gradient composition between 0% and 100% B.
To start the pump, click the Start button. Note the running man icon.
To change the pump parameters while the pump is running, enter the
new value and then click on the set button.
Window Help
1 wash
2 load
Report Manual Setup Protocol
Fraction Collector
Mode: System Local
Rack: Rack 1 (128 tubes)
Start Tube: 1
End Tube:
128
Fraction size:
1.00
ml
1
Tube number:
Volume left:
Advance
START STOP
2
3
UV
-0.00181 AU
4
Run Notes Log
PostRun
UV Detector
Zero Baseline
ON OFF
Chart Recorder
Conductivity
500
range(mS/cm):
UV range (AU):
0.1
Event mark
ON OFF
Signal Import Module
SIM 1 and SIM 2 connected
AVR7-3 at port 4 AVR9-8 at port 5
I
8
L P 7
6
Conductivity
47.5 mS/cm
ON
I
2
3
4
5
SIM1/SIG SIM2/pH
0.089 Volt 7.00 pH