BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 31

Starting the Run
1. Insure that sufficient tubes are in the fraction collector rack
(approximately 14) and that the drop-head is over tube 1.
2. Insure that the V7-3 valve is in the LOAD position (position L).
3. Insure that the 50 µl sample loop is connected to ports 3 and 6 of the
inject valve. Completely fill the loop with sample (anion exchange
protein standard) via port 5 and a syringe and needle. Do not remove
the syringe from the injection port after filling the loop or the sample
will siphon to waste.
4. Click on the green Start toolbar button to start the method. The alarm
will sound after 1 minute. At this time move the V7-3 valve to the
Inject position (i.e. move the arm to the left). The sample will be
loaded onto the column and the method will be advanced. You may
choose to leave the valve in the inject position for the duration of the
run or return it to load position after all the sample has been loaded.
5. To Hold, Pause or Abort a run, click on the appropriate toolbar button.
Clicking the Hold toolbar button will hold the gradient pumps at the
current %B value and will not advance the programmed Method until
the Continue toolbar button is pressed. Clicking the Pause toolbar
button will stop the pumps completely. Clicking the Continue toolbar
button will re-start the pumps at exactly the point where the program
was paused.
6. When this run is finished, the gradient pumps will automatically stop
and a run finished message will appear in the bottom right of the
status bar.
7. Figure 10 shows a typical run control screen and chromatogram for this
separation.
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