BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 15

The Run Control Screen
1. Use the toolbar buttons at the left-hand side of the screen to check
that the screen display ranges for UV (0.1 AUFS) and conductivity
(100 mS/cm) are correctly set and that the gradient pump pressure
limits are appropriate (set to 700 psi High and 20 psi Low).
2. If you were equilibrating the column while writing the method, you
will notice that the Status Bar is displaying the flow-rate and values
for UV and conductivity. If necessary, you may wish to zero the UV
trace by clicking on the Zero baseline button. This button may be
clicked at any time.
3. To scale the on-screen chromatogram display axes, use the scroll bars
located on the left and right axes of the chromatogram window.
Starting the Run
1. Insure that sufficient tubes are in the fraction collector rack
(approximately 14). The drophead will automatically move to tube 1
when the Run is started.
2. Insure that the AVR7-3 valve is in the LOAD position (position L). If
it is not, return to the Manual Screen by clicking the toolbar Manual
button and click on valve position L.
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