BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 12

1. Click on the Fraction Collector button in the Available Devices box.
A dialog box will appear asking you to choose the type of collector
i.e. a generic collector, a Model 2110 or a Model 2128. Click on
Model 2128 and click the OK button.
You will see 2128 Fraction Collector in the Devices in Setup box.
2. Next, click on the AVR7-3 Valve button in the Available Devices box.
You will see a dialog box with a pull-down menu under Valve
Name/Function. You should see Sample Inject already selected.
Other functions are available from the pull-down menu. This action
is called valve function aliasing. You will see that the valve position
names are Load for position 1, Inject for position 2, and Purge for
position 3.
BioLogic Duo-Flow - Jennifer - System training - Starter Kit -
File
Edit View Utilities
New Edit New
Browser
Method Method Run
Available Devices
Aux Load
Pump
UV
Detector
SIM1 SIM1
1 1
pH Signal
SVT3-2
SV3
Valve
AVR7-3
P
U
Valve M
P
INJECT
Gradient Pump: F-10
Inlet A:
Inlet B:
Gradient Pump: F-10
0 %B 438 psi
0.00ml/min
Fig. 3. Setup editor.
3. Next, go to the box labeled Gradient Pump. Use the tab key or click
and drag on the field buffer A to highlight it and type in 25 mM Tris,
pH 8.1. Use the tab key to highlight buffer B and type 25 mM Tris +
0.5 M NaCl, pH 8.1. This operation is called aliasing the gradient
pump inlets.
Options Window Help
1 wash
2 load
Report Manual Setup Protocol
Fraction
Collector
Conductivity
Monitor
SIM2 SIM2
2 2
pH Signal
SV5-4
Valve
AVR9-8
Valve
25 mM Tris
25 mM Tris + 0.5 M NaCl
UV
-0.00284 AU
10
Run Notes PostRun Log
Devices in setup
2128 Fraction Collector
UV Detector
Conductivity Monitor
SVT3-2 Valve - Fraction Collector Diverter
Conductivity
59.0 mS/cm
Delete
Port 1
SIM1/SIG SIM1/pH
0.004 Volt 7.00 pH