BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 13

4. The Setup Editor is now complete and you are now ready to program
the separation steps. To see the protocol editor, click on the Protocol
Editor toolbar menu button.
Programming the Protocol Editor
1. From the Options pull-down menu, insure that Use Volume (ml) is
selected, so that the programming base is Volume.
BioLogic Duo-Flow - Dr. J. Smith - Starter Kit - UNO Q1 -
Edit View Utilities
File
New Edit New
Browser
Method Method Run
Add Step
Volumn
Isocratic
Flow
1 0.0
Load/Inject
2 0.0
Sample
Linear
3 0.0
Gradient
Change
4 1.0
Valve
5
1.0
Hold
6 1.5
Alarm
2.3
7
Zero
Baseline
15.3
8
UV Lamp
9 18.1
Chart
Recorder
26.1
Repeat
Steps
Fraction
Collection
Gradient Pump: F-10
0 %B 1 psi
0.00ml/min
Enter volume of sample to load
Fig. 4. Protocol editor.
2. Program the separation method listed below and in Figure 4.
• From the left side of the screen, press the Fraction collection
button. In the pop-up window that appears, choose Collect All
with a fraction size of 2.00 ml and a delay of 0. Make sure that you
choose the correct rack number for the rack you will be using
with the Model 2128.
• Program the remaining steps using the add step icons from the
left-hand side of the screen.
Options Window Help
1 wash
2 load
Report Manual Setup Protocol
Description
Collect Fractions of size 2.00 ml during entire run
Chart Recorder
Isocratic Flow
Set Zero Baseline
Load/Inject Sample
Isocratic Flow
Linear Gradient
Isocratic Flow
Isocratic Flow
End of Protocol
UV
0.00375 AU
11
Run Log
Notes PostRun
Parameters
Turn ON
100%
25mM tris
25mM tris, 0.5M NaCl,
0%
UV Detector
Load: Sample
Manual Inject Valve
Static Loop
25mM tris 100%
0%
25mM tris, 0.5M NaCl,
25mM tris 100% --> 50%
25mM tris, 0.5M NaCl, 0% --> 50%
0%
25mM tris
25mM tris, 0.5M NaCl, 100%
25mM tris 100%
25mM tris, 0.5M NaCl,
0%
Conductivity
3.59 mS/cm
Cut Copy Paste Delete
Volume: 1.0 ml
Flow: 4.00 ml/min
Volume: 0.5 ml
Flow: 4.00 ml/min
Volume: 0.8 ml
Flow: 4.00 ml/min
Volume: 13.0 ml
Flow: 4.00 ml/min
Volume: 2.8 ml
Flow: 4.00 ml/min
Volume: 8.0 ml
Flow: 4.00 ml/min
SIM1/SIG SIM1/pH
-0.003 Volt