BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 29

Step (ml)
Number Start
1. 0.0
2. 0.0
3. 0.0
4. 1.0
5. 1.0
6. 1.5
7. 2.3
8. 15.3
9. 18.1
10. 26.1
3. When you have finished with the protocol editor, click on the toolbar
button RUN. You will see a dialog box asking you to name the run.
For now, type in Run 1 and click the OK button. You will now see the
Run Control Screen (see Figure 10).
Step
Collect fractions of size 2.00 ml during entire run
Turn chart recorder ON
Isocratic Flow with 100% 25 mM tris, pH 8.1,
0% 25 mM tris, 0.5 M NaCl, pH 8.1 at 4.00 ml/min
for 1.0 ml
Set UV baseline to 0.0
Static loop: Inject 0.5 ml sample at 4.00 ml/min for
0.1 min
Isocratic flow with 100% 25 mM tris, pH 8.1,
0% 25 mM tris, 0.5 M NaCl, pH 8.1 at 4.00 ml/min
for 0.8 ml
Linear gradient with 0% to 50% 25 mM tris
0.5 M NaCl, pH 8.1 at 4.00 ml/min for 13.0 ml
Isocratic flow with 0% 25 mM tris, pH 8.1,
100% 25 mM tris, 0.5 M NaCl, pH 8.1 at
4.00 ml/min for 2.8 ml
Isocratic flow with 100% 25 mM tris, pH 8.1,
0% 25 mM tris, 0.5 M NaCl, pH 8.1 at 4.00 ml/min
for 8.0 ml
End of protocol
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