BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 20

• Continue to pump water through the system for at least 10 minutes.
You may wish to continue fraction collection or return the
SV3-2 valve to position 1 and then stop fraction collection.
Note: In manual operation, starting or stopping fraction collection
does not change the SV3-2 diverter valve position. You must always
manually set the SV3-2 valve to the waste or collect position.
b. Using a Model 2128 Fraction Collector.
The Model 2128 Fraction Collector has two operating modes:
• System: Controlled by the BioLogic HR System.
• Local: Controlled from its own faceplate.
For now, insure that the System button is selected.
When in System mode, operation of the fraction collector is as follows.
• Control of the Model 2128 is identical regardless of whether or not
the optional on-arm diverter valve is used. Without a valve, the
drop-head returns to the waste trough during divert operations.
• The fraction collector box on the manual screen will show fields
for Rack type, Start tube, End tube, Fraction size–ml, Tube
number, Volume left, a toggle button for Start and Stop and a
button for Advance (see Figure 7).
BioLogicHR - Default - no method
File View Utilities Options
New Edit New
Method Method Run Browser
Gradient pump
Flow rate:
1.00 ml/min
Inlet A: 100 %
%
0
Inlet B:
High
psi
1000
limit:
Low
0 psi
limit:
Set
START STOP
SV3-2 at port 1 SV5-4 at port 2
1
1 2
4
Gradient Pump
0.00 ml/min
0 %B 438 psi
Flowrate of the gradient pump
Fig. 7. Manual Control Screen for the Automated BioLogic System.
Window Help
1 wash
2 load
Report Manual Setup Protocol
Fraction Collector
Mode: System Local
Rack: Rack 1 (128 tubes)
Start Tube: 1
End Tube:
128
Fraction size: 1.00
ml
1
Tube number:
Volume left:
Advance
START STOP
2
3
UV
-0.00181 AU
19
Run Notes Log
PostRun
UV Detector
Zero Baseline
ON OFF
Chart Recorder
Conductivity
500
range(mS/cm):
UV range (AU):
0.1
Event mark
ON OFF
Signal Import Module
SIM 1 and SIM 2 connected
AV7-3 at port 4 AV9-8 at port 5
I
8
L P 7
6
Conductivity
47.5 mS/cm
ON
I
2
3
4
5
SIM1/SIG SIM2/pH
0.089 Volt 7.00 pH