Creating A Method For The Automated Biologic Hr System - BIO RAD BioLogic Duo-Flow Starter Kit Instructions

4.7 Creating a Method for the Automated
BioLogic HR System
From the Manual Control Screen, click the New Method toolbar
button. You will see a dialog box asking for a method name (input Starter
Kit Separation–Auto). Use the keyboard tab key to go to the Method
Description and Method Author fields. Make an entry in these fields if
desired and click the OK button. You will now see the Setup Editor.
At the chart recorder, set the Chart Speed at 60 cm/hr.
Note: Chart speed is not controlled at the BioLogic HR system.
Programming the Setup Editor
This information entered in the Setup Editor (see Figure 11) is
important for the subsequent separation method. The buttons grouped in
the left hand box (Available Devices) shows all the devices that could
theoretically be connected to the BioLogic HR System.
The list of items in the right hand box (Devices in Setup) identifies
those devices you wish to use in the subsequent method. The initial default
Devices in Setup are a UV detector and a conductivity monitor as these
come as standard with the BioLogic HR System. The default devices in
Setup can be changed by choosing Save setup as default from the Data
pull-down menu.
1. Click on the fraction collector button in the available devices box. A
dialog box will appear asking you to choose the type of collector
i.e. a generic collector, a Model 2110 or a Model 2128. Click on
Model 2128 and click the OK button.
You will now see 2128 Fraction Collector in the devices in setup box.
2. Next, click on the AV7-3 Valve button in the Available Devices box.
You will see a dialog box with a pull-down menu under Valve
Name/Function. You should see Sample Inject already selected.
Other functions are available from the pull-down menu. This action
is called valve function aliasing. You will see that the valve position
names are Load for position 1, Inject for position 2, and Purge for
position 3.
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