BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 34

Programming the Protocol Editor
1. From the Options pull-down menu, insure that Use Volume (ml) is
selected, so that the programming base is Volume.
BioLogicHR - Dr. J. Smith - Starter Kit - UNO Q1 -
Edit View Utilities
File
Edit
New New
Method Method Run Browser
Add Step
Volumn
Isocratic
Flow
1 0.0
Load/Inject
2 0.0
Sample
Linear
3 0.0
Gradient
Change
4 1.0
Valve
5
1.0
Hold
6 1.5
Alarm
2.3
7
Zero
Baseline
15.3
8
UV Lamp
9 18.1
Chart
Recorder
26.1
Repeat
Steps
Fraction
Collection
Gradient Pump
0 %B 1 psi
0.00ml/min
Enter volume of sample to load
Fig. 12. Protocol editor for an Automated BioLogic System.
2. Program the separation method listed below and in Figure 12.
• From the left side of the screen, press the Fraction Collection button.
In the pop-up window that appears, choose Collect All with a fraction
size of 2.00 ml and a delay of 0. Make sure that you choose the correct
rack number for the rack you will be using with the model 2128.
• Program the remaining steps using the add step icons from the
left side of the screen.
Options Window Help
1 wash
2 load
Report Manual Setup Protocol
Description
Collect Fractions of size 2.00 ml during entire run
Chart Recorder
Isocratic Flow
Set Zero Baseline
Load/Inject Sample
Isocratic Flow
Linear Gradient
Isocratic Flow
Isocratic Flow
End of Protocol
UV
0.00375 AU
33
Run Notes Log
PostRun
Parameters
Turn ON
100%
25mM tris
25mM tris, 0.5M NaCl,
0%
UV Detector
Load: Sample
Manual Inject Valve
Static Loop
25mM tris 100%
0%
25mM tris, 0.5M NaCl,
25mM tris
100% --> 50%
25mM tris, 0.5M NaCl, 0% --> 50%
0%
25mM tris
25mM tris, 0.5M NaCl, 100%
25mM tris 100%
25mM tris, 0.5M NaCl,
0%
Conductivity
3.59 mS/cm
Copy
Cut Paste Delete
Volume: 1.0 ml
Flow: 4.00 ml/min
Volume: 0.5 ml
Flow: 4.00 ml/min
Volume: 0.8 ml
Flow: 4.00 ml/min
Volume: 13.0 ml
Flow: 4.00 ml/min
Volume: 2.8 ml
Flow: 4.00 ml/min
Volume: 8.0 ml
Flow: 4.00 ml/min
SIM1/SIG SIM1/pH
-0.003 Volt