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Helios Gene Gun System
bio-rad Helios Gene Gun System Manuals
Manuals and User Guides for bio-rad Helios Gene Gun System. We have
1
bio-rad Helios Gene Gun System manual available for free PDF download: Instruction Manual
bio-rad Helios Gene Gun System Instruction Manual (60 pages)
Brand:
bio-rad
| Category:
Laboratory Equipment
| Size: 1.66 MB
Table of Contents
Table of Contents
3
General Safety Information
5
Helios Gene Gun Safety
5
Pressurized Helium and Nitrogen Safety
5
Power Safety
6
Ear and Eye Protection
6
Introduction to Particle Delivery
7
Particle Delivery Technology
7
The Helios Gene Gun
7
Operating Principle of the Helios Gene Gun System
8
Requirements for System Operation
9
User Supplied Components
10
Product Description
12
Packing List
12
Section 3 Product Description
12
Identification of System Components and Controls
14
Inserting the Battery into the Helios Gene Gun
18
Connecting the Helios Gene Gun to a Helium Source
19
Setting up the Tubing Prep Station
20
Operation of the Helios Gene Gun System
23
Quick Guide to Operation
23
Preparation of System Components Prior to Bombardment
24
Particle Delivery Using the Helios Gene Gun System
30
Removing Used Cartridges, Depressurization, and Shutdown
35
Preparation of Mammalian Cell Targets
36
In Vitro Delivery to Adherent Cells
36
In Vitro Delivery to Suspension Cultures
37
In Vivo Delivery to Epidermis
39
Optimization of Gene Gun Parameters
40
Overview
40
Parameters for in Vitro Delivery
42
Parameters for in Vivo Delivery
43
Troubleshooting
43
Dna/Microcarrier Preparation
43
Cartridge Preparation
43
Helios Gene Gun Operation
44
In Vitro and in Vivo Targeting
44
Product Information
45
Section 9 Product Information
45
Spare Parts
46
Gene Gun Specifications
46
Tubing Prep Station Specifications
47
Appendices
47
Precipitation of RNA Onto Microcarriers
47
Replacing the O-Rings and Barrel Liner on the Helios Gene Gun
48
Replacing the O-Ring on the Tubing Prep Station
49
Replacement of Razor Blade on and Disassembly of the Tubing Cutter
49
Cleaning and Sterilizing the Helios Gene Gun
51
Testing Cartridges for Microcarrier Penetration and Density (Optional)
51
Quantification of DNA in Cartridges
54
References
55
Quick Guide to Operation
56
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