BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 5

Section 1
Preparation for Use of the
Duo-Flow System
When the BioLogic Duo-Flow System is turned on, the Manual Control
Screen is displayed. (see Figure 1) This screen consists of a series of
boxes containing instrument faceplates which provide direct control of
the gradient pumps, a fraction collector, the UV and conductivity detector
range settings and valves.
1. Priming the BioLogic Duo-Flow Gradient Pumps.
a. Immerse the gradient pump A and B inlet lines in a container of
b. Connect the syringe (supplied with the fittings kit) to the priming
c. Turn the priming port counter-clockwise to open the seal. Gently
d. Repeat this operation several times until no air bubbles are visible
e. Tighten the priming port by turning it clockwise.
f.
2. Moving the Inject Valve to Purge Position.
To change the status of an automated inject valve AVR7-3, go to the
Manual Screen displayed on the controller monitor.
If you plugged an inject valve into port 4, you will see a valve box
designated AVR7-3 at port 4. The three radio buttons of this box
correspond to valve positions as follows: L = Load position, I = Inject
position, P = Purge position.
To move the AVR7-3 valve to Purge position, click button P.
Note: The default position at power up and at the end of a programmed
method for the AVR7-3 is L. For all other automated valves the default
is position 1.
HPLC grade (filtered, degassed) or other high quality water.
port of pump head A.
withdraw the syringe plunger to draw water into the pump head.
in the inlet tubing.
Repeat this priming procedure for pump head B.
3