BIO RAD BioLogic Duo-Flow Starter Kit Instructions page 37

BioLogicHR - Carole Smith - Uno Q1 data - Uno Q1 - Standards
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Method Method Run
UV
Frac. Collector
Advance
0.100
Divert Valve
0.090
Collect
Waste 0.080
Grad. Pump
0.070
High psi
1000
0.060
Low psi
0.050
0
Set
0.040
Chart Recorder
0.030
UV Range
0.01
0.020
Cond Range
0.010
500
0.000
Event
mark
-0.010
UV Detector
00:00:00
AU
Zero
Baseline
Protocol: > 1 0:00.0 Isocratic Flow with 100% Buffer B at 1.00 ml/min for 25.0 min
Run Time Run Volume
0:00.0
Gradient Pump
0.00ml/min 0 %B
Fig. 13. Run control screen showing a completed run on an Automated
BioLogic HR System.
3. Insure that the 50 µl sample loop is connected to ports 3 and 6 of the
inject valve. Completely fill the loop with sample protein standard
via port 5 and a syringe and needle. Do not remove the syringe from
the injection port after filling the loop or the sample will siphon to
waste.
4. To launch the Run, click on the green Start toolbar button. The sample
will be loaded automatically.
5. Clicking the Hold toolbar button will hold the gradient pumps at the
current %B value and will not advance the programmed method until
the Continue toolbar button is pressed. Clicking the Pause toolbar
button will stop the pumps completely. Clicking the Continue toolbar
button will re-start the pumps at exactly the point where the program
was paused.
6. When this run is finished, the pumps automatically stop and a run
finished message appears in the bottom right of the status bar.
7. Figure 13 shows a typical run screen and chromatogram for this
separation.
Window Help
1 wash
2 load
Report Manual Setup Protocol
1 2 3 4 5
100% Buffer B
50.0
00:02:00
StepTime Left
0.0 ml 0:24.9
UV
0 psi 0.00003 AU
36
Run Notes Log
PostRun
Fractions
6 7 8 9 10
00:04:00
Hr:Min:Sec
Fraction Vol. Left
Conductivity
-0.014 mS/cm
FullView
Conductivity
11 12 13
50.0
45.0
40.0
35.0
30.0
25.0
20.0
15.0
10.0
5.0
0.0
-5.0
00:06:00
mS/cm
Valve Info
SIM1/SIG SIM1/pH