Bio-Rad DCODE Manuals

Manuals and User Guides for Bio-Rad DCODE. We have 1 Bio-Rad DCODE manual available for free PDF download: Manual

Bio-Rad DCODE Manual

Bio-Rad DCODE Manual (85 pages)

UNIVERSAL MUTATION DETECTION SYSTEM 170-9080;170-9104

Brand: Bio-Rad | Category: Laboratory Equipment | Size: 0 MB

Table of Contents

Table of Contents
3
General Safety Information

5
- Caution Symbols
  5
- Precautions During Set-Up
  5
- Precautions During a Run
  5
- Precautions after a Run
  6
- Safety
  6
Introduction

6
- Introduction to Mutation Detection Technology
  6
Product Description

6
- Packing List
  6
- System Components and Accessories
  9
Denaturing Gel Electrophoresis (DGGE, CDGE, TTGE)

15
Heteroduplex Analysis

45
- Introduction to Heteroduplex Analysis
  45
- Section 5 Heteroduplex Analysis
  45
- Reagent Preparation
  46
- Gel Volumes
  48
- Sample Preparation
  49
- Temperature Controller
  49
- Adding the Running Buffer
  49
- Assembling the Heteroduplex Analysis Gel Sandwich
  50
- Casting Heteroduplex Analysis Gels
  51
- Single-Stranded Conformational Polymorphism (SSCP)
  
  52
  - Introduction to SSCP
    52
  - Reagent Preparation
    54
  - Gel Volumes
    56
  - Sample Preparation
    56
  - Temperature Controller
    56
  - Cooling the Running Buffer and Chiller Settings
    57
  - Assembling the SSCP Gel Sandwich
    57
  - Casting SSCP Gels
    59
- Protein Truncation Test (PTT)
  
  60
  - Introduction to PTT
    60
  - Section 7 Protein Truncation Test (PTT)
    60
  - Reagent Preparation
    61
  - Gel Volumes
    63
  - Sample Preparation
    63
  - Temperature Controller
    63
  - Adding the Running Buffer
    64
  - Assembling the PTT Gel Sandwich
    64
  - Casting PTT Gels
    66
- Electrophoresis
  
  67
  - Assembling the Upper Buffer Chamber
    67
  - Section 8 Electrophoresis
    67
  - Sample Loading
    69
  - Running the Gel
    69
  - Removing the Gel
    71
  - Staining and Photographing the Gel
    72
- Troubleshooting Guide
  
  73
  - Equipment
    73
  - Applications
    75
Specifications

79
- Section 10 Specifications
  79
Maintenance

80
References

80
- Section 11 Maintenance
  80
Instruments and Reagents for Mutation Detection Electrophoresis

81

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