Failure To Prime - BIO RAD Bio-Plex Pro Instructions For Use Manual

Perform priming for both channels using the following procedure:
Repeat for channel 2.

Failure to Prime

If you have a new instrument or it has not been used for a long time the pump may
fail to draw fluid from the liquid bottles. This problem is easily solved.
Alternately you may carefully use a syringe to inject 1 - 2 ml of water directly into the
L1 port of the instrument, reconnect the tubing, and run a prime procedure.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations
PROCEDURE
< > OK
PRIME PRIME
< < > > OK OK
PRIME SOLUTION OK?
< > YES
WASTE BOTTLE OK?
< > YES
VOLUME
< > OK
VOLUME 30 mL
+ - OK
CHANNEL 1
- + EXIT OK
PROGRAM
< > OK
1. Disconnect the L1 tube from the bottle (leaving it connected to the
instrument).
2. Hold the free end of the tube high and pipette 3 - 4 ml of deionized
water to the tube while performing the prime procedure in channel 1
as described above.
Basic Operation and Maintenance
Navigate to the Procedure in the primary
menu and press OK
Select and press OK for PRIME
Confirm rinsing solution and tube in bottle
Confirm waste bottle is connected and
sufficiently empty
Press OK if you see VOLUME. If you see
TIME, scroll down to VOLUME, then press OK
Press OK
Select CHANNEL 1 using the choice keys (< , >)
Select OK and the prime procedure starts
Instrument will perform the prime step and will
then be ready to perform wash protocols
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