Aspirating Modes; Normal Aspirationmode; Crosswise Aspiration Mode - BIO RAD Bio-Plex Pro Instructions For Use Manual

Defining New Programs

Aspirating Modes

To improve wash efficiency and reduce the residual volume, the manifold must be
positioned so that the aspirating needles are correctly positioned in the wells for
round-bottom, V-shaped bottom, or flat-bottom well microplates.

Normal AspirationMode

For round-bottom or V-shaped bottom wells, the aspirating needles are placed in the
middle of the wells. Only one aspiration position can be selected.
Normal Aspirating Positions

Crosswise Aspiration Mode

With flat bottom wells, the instrument can perform crosswise aspiration using two
aspiration positions per well.
The aspirating needles are set at two positions on the bottom of the wells
(front edge and back edge).
Crosswise Aspiration for Flat Bottom Wells
50
Round Bottom Wells
Aspirate Y Offset 1
Front edge
'V' Shaped Wells
Aspirate Y Offset 2
Back edge
Bio-Plex Pro and Bio-Plex Pro II Wash Stations