BIO RAD Bio-Plex Pro Instructions For Use Manual page 24

Basic Operation and Maintenance
Important Vacuum Protocol Precautions
•
Carefully place the plate so that it is flat and squarely seated between the metal frames on the
vacuum carrier before stating a vacuum protocol
•
It may be necessary to apply a small amount of pressure to the plate for the first vacuum cycle of the
day
•
Monitor your plate periodically to ensure complete removal of wash buffer before the next dispense
cycle
•
Blot the bottom of the filter plate on a paper towel within 5 seconds after each program is completed.
If you are not sure how much time has elapsed, run a PREVAC program to clear any residual buffer
that may have wicked back into the wells before proceeding to the next step
Vacuum protocols will not work if you do not have buffer in every well.
24 Bio-Plex Pro and Bio-Plex Pro II Wash Stations