BIO RAD Bio-Plex Pro Instructions For Use Manual page 52

Defining New Programs
Example of a typical wash program for working with adherent cells
52
DEFINE EDIT
< > EXIT OK
PROGR 1:
- + EXIT OK
NAME:CELLW ASH
567890, , ABCDE
PLATE MODE
- + EXIT OK
PLATE 1, GRE96ft
- + EXIT OK
NO. OF CYCLES 2
- + EXIT OK
C1 P1 W ASH
- + EXIT OK
POS: MOVE CUST.
- + EXIT OK
Z-User Def 7000
- + EXIT OK
VOLUME 450µl
- + EXIT OK
CHANNEL 1
- + EXIT OK
H-SPEED 1mm/s
- + EXIT OK
FLOWRATE DRIP
- + EXIT OK
PROGRAM END?
- + EXIT OK
FINAL ASP
- NO YES
To define a program press OK to enter the DEFINE EDIT
menu.
Select an appropriate position for the program.
Select an appropriate name for the program.
Select PLATE MODE and press OK.
Select an appropriate plate type and press OK.
Select 2 cycles and press OK.
Select WASH and press OK.
Select MOVE CUST. and press OK to continue to the next
parameter
Set the user defined Z-position to the appropriate height and
press OK to continue to the next parameter
Set the volume to 450 µl and press OK to continue to the next
parameter
set the channel to 1 and press OK to continue to the next
parameter
Set the head speed to 1 mm/s and press OK to continue to the
next parameter
Set the flow rate to DRIP and press OK to continue to the next
parameter
Select PROGRAM END? and press OK.
When the final aspiration step appears, press NO to finish
defining the program.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations