Metrohm 930 Compact IC Flex ChS/Deg Manual page 95

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Problem
The retention times
in the chromato-
gram have changed
unexpectedly.
Peak areas are lower
than expected.
Data of the separa-
tion column cannot
be read.
930 Compact IC Flex ChS/Deg (2.930.1260)
Cause
Guard column – Blocked.
Separation column –
Blocked.
Injection valve – blocked.
Eluent - Incorrect concen-
tration
Separation column –
Diminished separating effi-
ciency.
The eluent contains gas
bubbles.
High-pressure pump –
Defective.
Sample – There is a leak in
the sample path.
Sample – The sample path
is blocked.
Sample – The sample loop
is not (completely) filled.
Sample – There are gas
bubbles in the sample.
Column chip dirty.
Column chip is defective.
Remedy
Note: 6.2821.180 pump tubing connection
with filter must be used.
Replace the guard column (see Chapter 3.18,
page 44).
Regenerate the separation column (see
￭
Chapter 5.14.4, page 84).
Replace the separation column (see "Con-
￭
necting the separation column", page 48).
Note: Samples should always be microfiltered .
Have the valve cleaned (by a Metrohm service
engineer).
Create eluent with correct concentration.
Regenerate the separation column (see
￭
Chapter 5.14.4, page 84).
Replace the separation column (see "Con-
￭
necting the separation column", page 48).
Ensure that the connectors for the eluent
￭
degasser are firmly connected .
Deaerate the high-pressure pump (see Fig-
￭
ure , page 43).
Request Metrohm Service.
Find and fix the leak in the sample path.
Find and eliminate the blockage in the sample
path.
Adjust the transfer time for the sample (see
"Determining the transfer time", page 82).
Use the sample degasser .
Clean the contact surfaces of the column chip
with ethanol.
1. Save column configuration in MagIC Net.
6 Troubleshooting
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