Separation Column; Separating Efficiency; Protecting The Separation Column; Storing The Separation Column - Metrohm 930 Compact IC Flex ChS/Deg Manual

5.14 Separation column

5.14 Separation column
5.14.1

Separating efficiency

5.14.2

Protecting the separation column

5.14.3

Storing the separation column

5.14.4

Regenerating the separation column

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84
The analysis quality that can be attained depends in a large part on the
separating efficiency of the separation column being used. The separating
efficiency of the selected separation column must be sufficient for the cur-
rent analysis problems. If difficulties arise, start by checking the quality of
the separation column in each case by recording a standard chromato-
gram.
You can find detailed information on the separation columns available
from Metrohm in the leaflet provided along with your separation column,
in the Metrohm IC Column Program (available from your Metrohm
representative) or on the Internet at
Chromatography product area. You can find information on special IC
applications in the corresponding "Application Bulletins" or "Applica-
tion Notes", which are available on the Internet at
www.metrohm.com in the Applications area or free of charge upon
request from your responsible Metrohm representative.
We recommend using the following protective measures so that the sepa-
ration column retains its separating efficiency for as long as possible:
Microfilter both the sample and the eluent (0.45 µm filter) and aspirate
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the eluent via the aspiration filter (6.2821.090) as well.
Always use a guard column. Information regarding which guard col-
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umn is suitable for your separation column can be found in the Met-
rohm IC Column Program (which is available from your Metrohm
representative), the leaflet provided along with your separation column
or the product information about the separation column at
www.metrohm.com
obtained directly from your representative.
Use the pulsation absorber.
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Always store separation columns you do not need in a sealed and filled
state according to the column manufacturer's specifications.
The separation column can be regenerated according to the column man-
ufacturer's specifications if the separation characteristics of the column
have deteriorated. You can find information on regenerating separation
columns available from Metrohm on the leaflet provided with every col-
umn.
http://www.metrohm.com
(Ion Chromatography product area), or it can be
930 Compact IC Flex ChS/Deg (2.930.1260)
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in the Ion
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