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The display is almost the same for each image in the series and by default starts at image 1.
10.
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The Capture Interval controls are simple + / - buttons for adjusting the following time periods;
hours (h) , minutes (m) , seconds (s) , and milliseconds (ms) .
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Using the Capture Interval + / - buttons enter an interval time for the series of images.
13.
The manual Exposure controls are simple + / - buttons for adjusting the following time periods;
hours (h) , minutes (m) , seconds (s) , and milliseconds (ms) .
To set a manual exposure time select the Manual button and use the Exposure + / - buttons
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enter an exposure time for the first image. Alternatively, selecting the Auto button after you
have selected the lighting and filters allows the system to automatically select the most
appropriate exposure time for the image 1.
Use the Lighting and Filters functions in the right hand pane to make your lighting and filter
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selections as described previously.
Select the Next arrow
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apply the settings you have entered for image 1 to all of the remaining images in your series.
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By default the Same as previous checkbox is checked, making the settings for the second
image the same as the settings for first image. To make changes for the second and
subsequent images, uncheck the Same as previous checkbox.
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Enter the settings for all the images in your series.
Select the Next arrow
19.
If the Neutral Fielding option is available, determined by your lighting/filter selections, you now
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have to decide whether or not to apply Neutral Fielding.
The Neutral Fielding option allows you to correct the image for uneven lighting. This can cause
problems when quantifying bands or spots across a large sample. The image of the neutral
field is normalised for light illumination. This normalisation is then applied to the gel image,
such that uneven light illumination generated by the light source is addressed.
The exposure/lighting/filter choices that you have selected are now displayed as a set of
Current Protocols in the right hand pane.
Position the sample and improve the preview sample image as much as possible using the
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Light / Digital Zoom / Grid / Select Autoexpose Area / Lens Controls, as described previously.
Select the Capture button
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Instruction manual
to make the settings for image 2. Select the Last arrow
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ADDITIVE SERIES PROTOCOLS
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