VWR avantor Chemi Premium Instruction Manual
  1. Manuals
  2. Brands
  3. VWR Manuals
  4. Laboratory Equipment
  5. avantor Chemi Premium
  6. Instruction manual

VWR avantor Chemi Premium Instruction Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Instruction Manual
®
VWR
Gel Documentation Imager Chemi Premium
EUROPEAN CATALOGUE NUMBER: 730-1469
Version 01 Issue
04/2021

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the avantor Chemi Premium and is the answer not in the manual?

Questions and answers

Related Manuals for VWR avantor Chemi Premium

Summary of Contents for VWR avantor Chemi Premium

  • Page 1 Instruction Manual ® Gel Documentation Imager Chemi Premium EUROPEAN CATALOGUE NUMBER: 730-1469 Version 01 Issue 04/2021...

  • Page 2: Legal Address Of Manufacturer

    Legal address of Manufacturer Europe ® International bv, Researchpark Haasrode 2020 Geldenaak sebaan 464 B-3001 Leuven +3216385011 http:be.vwr.com UK Importer: VWR International Ltd Hunter Boulevard, Magna Park Lutterworth, Leicestershire, LE17 4XN http://uk.vwr.com Country of origin ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 3: Table Of Contents

    Lighting and Filter options to improve sample positioning Automatic image capture- Image Actions Following Capture Manual Image Capture Mode Manual Image Capture - Start Manual Image Capture - Single Image Manual Image Capture - Series ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 4 Saving Images Printing Operator Maintenance Looking After a Imager Chemi Premium Instrument Cleaning a Imager Chemi Premium Instrument User Replacable accessories and spare parts Contacting VWR International Imager Chemi Premium Troubleshooting Disposal Disposing of a Imager Chemi Premium ® vwr.com...

  • Page 5: Safety Information

    Safety Practices This document describes the general safety practices and precautions that must be observed when ® operating a VWR Gel Documentation Imager2 System. This advice is intended to supplement, not supersede, the normal safety codes in the user’s country. The information provided does not cover every safety procedure that should be followed.
  • Page 6 40°C.  Electrical supply fluctuations not exceeding +10% of the nominal voltage. The protection provided by the equipment may be impaired if the environmental conditions do not lie within these parameters. WARNING ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 7  It has any signs of visible damage.  If it has been subjected to prolonged storage in unfavourable conditions.  If it has been subjected to severe stress during transportation. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 8 UV Safety The Imager Chemi Premium System uses epi mid wave UV tubes and UV transilluminator. If the door is opened the image capture software will automatically switch off the UV light. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 9 To prevent potential personal injury and damage to the instrument, switch OFF all components in the system and disconnect them from the mains power supply before altering, or making any new electrical connections. WARNING ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 10: Unpacking A Imager Chemi Premium System

    ® Unpacking a VWR Imager Chemi Premium System Removing from the Packaging Visual Inspection Upon taking delivery of a new Imager Chemi Premium Instrument:  Check the contents of the cartons as they are unpacked against the contents listed on the packing list ...
  • Page 11 Open the cardboard carton top flaps Remove the carton and gently lift the Imager Chemi Premium out Attach the camera, slide the transilluminator into place, and put on the camera cover Imager Chemi Premium ready for use ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 12 (9-way D-type male to USB Type A) Pre-ordered Emission Filters Pre-ordered epi LED Modules (in pairs) Transilluminator UltraBright LED blue light transilluminator Visible light converter screen Black anti-reflective screen White light pad ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 13: Overview

    Gel Doc image capture software. Imager Chemi Premium system has a new generation of camera - having 4 million pixels, giving the system outstanding sensitivity and versatility. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 14: Imager Chemi Premium

    57 x 84 x 45 Weight (kg) Supply voltage (V ac) 115 / 240 other emission filters are available other wavelengths are available other sizes and wavelengths are available including dual wavelength ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 15: Gel Doc Image Capture Software

    The Gel Doc image capture software provides both automatic and manual modes of control of the Imager Chemi Premium hardware and has an inbuilt database of application specific data which it uses in ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 16 Sample type; Protein, DNA, RNA  Matrix type; Acrylamide, Agarose  Dye types  Lighting types CAMERA COVER INDICATOR LIGHTS DARKROOM DOOR DARKROOM DOOR MANUAL RELEASE BUTTON TYPICAL IMAGER CHEMI PREMIUM– FRONT ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 17 CAMERA MODULE INDICATOR LIGHTS ELECTROMAGNETIC DOOR CATCH UPPER LIGHT UNITS - BOTH SIDES TRANSILLUMINATOR UNIT TYPICAL IMAGER CHEMIN PREMIUM - DARKROOM DOOR OPEN ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 18: Software Installation

    Install Device Drivers using the Device Driver Installation Wizard Run the Set-up Program Insert the VWR USB stick and run the Set-Up Program. The following screen will appear: SETUP – Gel Doc - WELCOME TO THE Gel Doc SETUP WIZARD SCREEN Click on the Next >...

  • Page 19: Select The Instrument Hardware Information

    SETUP – Gel Doc - SELECT DESTINATION LOCATION SCREEN Select the folder in which you would like the Gel Doc image capture software to be installed:  To accept the displayed default folder click on the Next > button. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 20: Select The Start Menu Folder

    The Device Driver Installation Wizard screen will appear. Install Device Drivers using the Device Driver Installation Wizard This part of the installation process installs the software drivers that some computers need in order to complete the installation. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 21 This indicates that the installation process has finished successfully. SETUP – Gel Doc - COMPLETING THE Gel Doc SETUP WIZARD SCREEN Click on the Finish button to complete the Set-Up process. Remove the VWR USB stick. Your Gel Doc image capture software is now ready for use. ®...
  • Page 22 Windows 8, two levels of operator access are available; User and Administrator. User accounts have limited privileges within the Gl Doc image capture s software, Administrator accounts have full privileges. Check your desktop, you should have a Gel Doc icon visible, i.e. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 23: Operating

    Connect the Camera to PC lead to the USB Mini-B connector on the Camera. DO NOT connect to the PC. Energise the Darkroom - ON/OFF switch on the back of the Darkroom. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 24 G:BOX Instrument Imager Chemi Premium Instrument (rear) - Hood removed for clarity (rear)- Hood removed for clarity G:BOX INSTRUMENT - POWER AND IMAGER CHEMI PREMIUM- POWER AND DATA CONNECTIONS DATA CONNECTIONS ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 25: Operator Accessories

    Remove the Hood. Remove the Lens and Camera Units. The Filters are visible through an opening in the top of the Darkroom. FILTER VISIBLE ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 26: Switching Instrument On

    Switch on the instrument using the ON/OFF rocker switch on the rear of the Darkroom. Check that the green indicator light on the front of the Instrument lights. Gel Doc SOFTWARE LAUNCHED SCREEN Once the Gel Doc software has started up the Home screen will appear. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 27 Imager Chemi Premium with a 4MP camera is installed and the Gel Doc program is running. Displays the icon, for accessing System data - camera hardware and program version information, as shown below: ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 28 Manual Capture. Clicking on an icon takes the User to screen associated with the icon. When multiple steps are displayed a grey arrow appears in between each icon. This indicates each step back in ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 29: Entering Hardware Details

    Select the Hardware icon on the Home screen. This displays the Hardware screen. HARDWARE SCREEN Scroll the window vertically to view all available options. Select each hardware option installed in your Instrument. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 30 If you have pre-specified your exact requirements at time of order then this operation may have been performed by VWR and all the hardware may have been pre-installed. However, it is good practice to check that the hardware selections made on this screen match the actual hardware installed.

  • Page 31: Entering Personal Preferences

    Instrument settings, i.e. a profile, which reflects the nature of the work the individual normally undertakes and what they do with the test results Select the Settings icon on the Home screen. This displays the User Preferences screen. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 32 Sample Size- if you prefer not to see the sample size pop-up box when using the automated mode please un-check this checkbox.  Annotation Handle Size Defaults- checkbox to increase annotation handle size if using a touch screen. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 33 Displayed results only, incidence or quantity calibration results Export includes headers LOGGING DEFAULTS  Increase Logging level-this will permit VWR Support to access more information on how the software is running on your instrument. PRINTER SETTINGS Enables you to select a printer and set printer preferences for your selected printer, generally these will only be settable for the current session.
  • Page 34 If you select the Reset Dynamic Field Data button the following pop-up message is displayed: RESET DYNAMIC FIELD DATA MESSAGE POP-UP Select the Yes button to perform a Dynamic Fielding operation. Follow the onscreen prompts. Select the No button to cancel. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 35 Rapid then at the top right hand side a button will be available to change screen to chemi single image using the drop down menu.  Check the box to automatically invert chemi images- this will be a default setting. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 36 Alternatively, you can select the chemiluminescent reagent you wish to use as default by using the drop down menu. Chemi single settings Check box to image a visible or colour marker for chemi single. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 37 Select the number of images you wish to capture and whether you would like to add the contents of previous image. This will add the each captured image to the previous image. This will add each captured image to the previous image improving the signal to noise ratio. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 38 Enter default values for the exposure time settings for the signal accumulation feature for the first and last image. Select the number of images to capture for S.A.C as default. USER PREFERENCES SAVING DEFAULTS SETTINGS SCREEN ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 39 Image name settings- You can enter a name for each type of gel capture and for each type of blot image captured in Gel Doc image capture software to automatically use the entered name when exporting or saving that type of image. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 40: Adjusting Basic Screen Viewing Size

    Maximise/Restore Gel Doc button in the Title Bar and then resizing the display by dragging the dotted arrow icon in the bottom right hand corner. HOME SCREEN - DEFAULT HOME SCREEN - ADJUSTED ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 41: Recommended Light / Dye / Accessory And Other Application Tables

    If using an SP filter use a frosty DF screen on the Ultra-slim blue light LED Transilluminator then a blue DF screen. Epi-UV short and long wavelength light Use a blue DF screen on the Ultra-slim blue light LED Transilluminator. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 42: Loading Test Samples

    White Light Pad Installed The White light pad, if required to be used, should be installed in place of the UV Transilluminator. Place the sample centrally on the White light pad screen. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 43: Automatic Image Capture Mode

    Home screen. This displays the Gels drop-down list. GELS DROP-DOWN LIST Select your Gel type from the drop-down list e.g. DNA Agarose Gel. This displays a Sample Size pop-up dialogue box. SAMPLE SIZE POP-UP ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 44: Visble Protein Gel

    Note if you get the message ‘You do not have the hardware for this multiplex. To find out more contact VWR you may need to purchase additional lighting or filters to be able to image this dye. 4. On the sample positioning screen adjust the lens controls.

  • Page 45: Automatic Blot Image Capture

    On the sample positioning screen adjust the lens controls. Close the darkroom door and Gel Doc image capture software will automatically capture your image according to the chemi settings selected on the settings page. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 46: Chemi Blot (Single Image)

    Input the exposure time for each image or you can check the box to ‘use the same exposure for all images’. Adjust the lens controls to focus sample. Press the capture button to capture the images. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 47: Chemi Blot (Series)

    Note: If you get the message ‘you do not have the hardware for this multiplex. To find out more contact VWR. You may need to purchase a new lighting and filter option to be able to image this dye.

  • Page 48: Visble Blot

    Doc image capture software to remove the emission filter so it is no longer in front of the Camera. The sample image should now appear much clearer. The software will automatically place the emission filter back in front of the Camera when the green bouncing arrow is pressed. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 49 No Grid option removes the grid projection. Show Grid option displays the default grid projection, 2 rows x 2 columns. Gel Frame to use with stain free imaging for protein normalisation. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 50 Recommended Light / Dye / Accessory and Other Application Tables. In the Dynamic Fielding box check the Use Dynamic Fielding checkbox. Follow the onscreen messages. TYPICAL DYNAMIC FIELDING MESSAGES ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 51 CURRENT PROTOCOL BOX IMAGE CAPTURE To capture an image using the entered selections, select the Capture button The following pop-up message is displayed as the Instrument makes an image capture: AUTO EXPOSING MESSAGE POP-UP ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 52 If multiple images are captured without any images being saved, multiple images can be selected and saved in a single operation. Check the Multi Select Mode checkbox in the Image Pool and select multiple images. To save the selected images, select the Save icon ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 53 This gives the recorded image an overall darker or lighter appearance. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 54 Automatic Image Capture - Image Actions Following Capture Further actions can be performed on captured images by selecting Image Action icons. The function of these is explained in other parts of this User Guide. IMAGE ACTION ICONS ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 55: Manual Image Capture Mode

    To view the image capture options available select the Capture Setup menu . This displays the Image Capture options in a drop down menu. Select the type of image capture you want to make by selecting from the options in the drop down list. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 56: Manual Image Capture - Single Image

    Single Image button from the Image Capture options. MANUAL CAPTURE - SINGLE IMAGE SELECTED Use the Lighting and Filters functions in the right hand pane to make your lighting and filter selections. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 57 On initially calling up the Manual Capture screen the Dynamic Fielding function in the right hand pane will have been displaying the following; . Once you have made Lighting and Filters selections the Dynamic Fielding function will change to the following; ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 58 For details of Dynamic Fielding screens and lighting combinations refer to Recommended Light / Dye / Accessory and Other Application Tables. In the Dynamic Fielding box check the Use Dynamic Fielding checkbox. Follow the onscreen messages. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 59 LENS CONTROLS Note: The lens controls that are available are Focus, Iris (aperture), and Zoom. The available lens controls appear in the Lens Control pane. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 60 2 x 2 binning on an 8 MP sensor effectively results in a 2 MP sensor with pixels four times the size of the 8 MP sensor pixels. Select the Binning button and select the binning ratio you want to use from the drop-down list. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 61 Image Pool pane. If more images are captured, these also appear in the Image Pool. When initially captured, images in the Image Pool are unsaved and are outlined in red. UNSAVED IMAGE POOL IMAGE ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 62 The Exposure controls are simple plus/minus buttons for adjusting the following time periods; hours (h) , minutes (m) , seconds (s) , and milliseconds (ms) . MANUAL EXPOSURE CONTROLS Using the Exposure plus/minus buttons enter an exposure time for the image. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 63 If further images are captured, additional images appear in the Image Pool, stacked vertically. Images can be dismissed from the Image Pool by selecting them and then selecting the Close button . If an image has not been saved you will see a Save prompt. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 64: Manual Image Capture - Series

    Select the No Binning (9.13MP) button and select the binning ratio you want to use from the drop-down list, then select the Next arrow , or if you want to use the full resolution just select the Next arrow ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 65 The controls for these are displayed in the right hand pane. SERIES EXPOSURE CONTROLS - FIRST IMAGE SERIES EXPOSURE CONTROLS - SECOND IMAGE SAME AS PREVIOUS UNSET ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 66 The image of the dynamic field is normalised for light illumination. This normalisation is then applied to the gel image, such that uneven light illumination generated by the light source is addressed. For details of Dynamic ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 67 When the capture is complete a green tick appears inside the protocol box and the yellow highlight is removed. The yellow highlight then appears on the next protocol box in the series, once that capture has commenced. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 68 SAVED IMAGE POOL IMAGE Images can be dismissed from the Image Pool by selecting them and then selecting the Close button . If an image has not been saved you will see a Save prompt. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 69: Manual Image Capture - Additive Series

    Select the No Binning (9.13MP) button and select the binning ratio you want to use from the drop-down list, then select the Next arrow , or if you want to use the full resolution just select the Next arrow ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 70 For details of Dynamic Fielding screens and lighting combinations refer to Recommended Light / Dye / Accessory and Other Application Tables. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 71 The Current Protocol display changes as the series of captures is made. As a capture is made using a protocol, the protocol box is highlighted yellow. When the capture is complete a green ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 72 Images can be dismissed from the Image Pool by selecting them and then selecting the Close button . If an image has not been saved you will see a Save Images prompt. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 73: Manual Image Capture - Multiplex

    Select the No Binning (9.13MP) button and select the binning ratio you want to use from the drop-down list, then select the Next arrow , or if you want to use the full resolution just select the Next arrow ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 74 For details of Dynamic Fielding screens and lighting combinations refer to Recommended Light / Dye / Accessory and Other Application Tables. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 75 By default the main image is the selected image from the thumbnail stack, this is indicated by a yellow highlight around the top image in the stack. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 76 Select the right arrow at the side of the thumbnail image you want to change and select a new colour from the drop down menu. To reject a colour and return to the original setting select Default from the drop down menu. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 77 SAVED IMAGE POOL IMAGE Images can be dismissed from the Image Pool by selecting them and then selecting the Close button . If an image has not been saved you will see a Save prompt. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 78: Manual Image Capture - Image Actions Following Capture

    Manual Image Capture - Image Actions Following Capture Further actions can be performed on captured images by selecting Image Action icons. The function of these is explained in other parts of this User Guide. IMAGE ACTION ICONS ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 79: Protocols- Saving. Opening And Editing

    Follow the prompt in the pop-up and either select an existing Protocol from those displayed (which will be overwritten by you saving your new Protocol), or enter a name for your new Protocol in the Enter Protocol name here… box, e.g. Stain free gel. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 80 Once the image has been captured the Saved Protocols pop-up appears. SAVED PROTOCOLS POP-UP Select the Yes button. The Save Protocol pop-up appears. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 81: In Auto Image Capture Mode

    Work through the workflow for the type of test sample that you are going to image.Once the image has been captured the Saved Protocols pop-up appears. SAVED PROTOCOLS POP-UP Select the Yes button. The Save Protocol pop-up appears. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 82: In Manual Image Capture Mode

    To create a Protocol in Manual Image Capture mode: Work through the Manual Image Capture process described previously and capture an image. Select the Save Protocol icon from the Image Action icons. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 83: Using Protocols

    Opening a System Protocol System protocols are pre-installed configurations that the user can use for imaging some of the more common applications. To open and use a system Protocol from the Home screen: ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 84: Opening A Protocol

    Protocol file and will take you to the following point in the image capture process: If the Protocol is in Auto Image Capture mode the system will display the Sample Positioning screen, as shown below: A\UTOMATIC CAPTURE SAMPLE POSITIONING ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 85: Deleting A Protocol

    To edit a previously saved Protocol, first open the Protocol as described previously. Then use the normal controls/actions available on the Auto Image Capture Sample Positioning screen or Manual Capture screen to change the settings. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 86: Image Functions

    Images displayed in the central pane of the Load Images screen can be sorted by two parameters; Sort by Name or Sort by Date. Select one of the two icons at the top of the central image pane. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 87 Selecting an image or images in the central pane adds the selected image or images to the Selected Images pane. At the same time the image(s) selected in the central pane turn green, as shown below. SELECTED IMAGES ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 88: Image Actions From Load Images Screen

    The View Images screen can also be accessed from any other screen displaying the View Images icon in the Image Action icons. Note: Selected function buttons turn red. They stay red and the function remains active until the function button is re-selected. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 89: View Images Screen - Single Images

    Images are displayed as thumbnails on the View Images screen. The size of the images can be changed by adjusting the Zoom Slider control. ZOOM SLIDER CHANGE LAYOUT Two options are available using the Change Layout controls. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 90 Scroll bars allow you to view the entire area of an image. A horizontal scroll bar appears when the Zoom function is used on an image. The right hand pane contains the following controls:  The Change Layout controls.  The Link controls. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 91 If the graph reaches to the far right (65535 grayscales) this indicates that the image may be saturated. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 92 MANAGE IMAGES Two options are available using the Manage Images controls. MANAGE IMAGES CONTROLS Selecting the Close button closes only the selected image file(s). Selecting the Close All button closes all image files. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 93: View Images Screen - Combining Single Images Into A New Multiplexed Image

    . This displays the Create Multiplexed Image pop-up. This shows what the multiplexed image will look like. Two options are available using the Set Type of Composite Image buttons; Create Monochrome Image or Create Colour Image. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 94: Image Edit Screen

    3D View By default the Image Edit screen opens in General mode. Note: Selected function buttons turn red. They stay red and the function remains active until the function button is re-selected. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 95: Image Edit Screen - General Option

    Image Source  Rotation  Copy Image  Other Note: Selected function buttons turn red. They stay red and the function remains active until the function button is re-selected. GENERAL CONTROLS/FUNCTIONS IMAGE SOURCE ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 96 Select the Default button and select a new colour from the drop-down menu. To reject a colour and return to the original setting select Default from the drop down menu. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 97 Use the colour picker to add a custom colour to a single image. Select the ‘Colour Image’ button then from the pop up use the slider bars to create a custom colour. Press the ‘Reset Colour’ button to remove the custom colour. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 98: Image Edit Screen - Annotate Option

    The following image annotation tools are available: Select tool Polygon tool Pan tool Line tool Text tool Arrow tool Fill/No Fill tool Select Colour tool Rectangle tool Select Line Style tool Ellipse tool ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 99 'handles'. Shapes can be filled using the Fill/No Fill and Select Colour tools. The outline of the shape can be coloured and the style of the line can be changed using the Select Colour and Select Line Style tools. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 100: Image Edit Screen - Enhance Option

    The Delete key on the PC keyboard can also be used to delete selected annotations. Image Edit Screen - Enhance Option The lower left hand pane of the Enhance screen provides the following controls/functions:  Image Source  Enhancement Tools  Resolution ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 101 The Define area to be cropped tool enables you to select an area of the selected image so that you can isolate the chosen portion of the image from the rest of the image in order to view ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 102 Default image resolution New selection RESOLUTION MENU The options displayed in the Resolution drop-down menu depend on the resolution that the image is captured at, an example is shown below: ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 103: Image Edit Screen - General / Annotate / Enhance Options Common Controls

    The Gamma slider changes the tone of the overall image. With reference to the histogram mentioned previously, the Gamma function changes the relative brightness of the recorded midrange tones by shifting them either towards the dark end or the white end of the graph, ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 104 The histogram is a graph showing the number of pixels in the image at each different intensity value found in that image. For a 16-bit image there are a possible 65535 different intensities so ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 105: Image Edit Screen - 3D View Option

    Selected function buttons turn red. They stay red and the function remains active until the function button is re-selected. 3D VIEW OPTION CONTROLS/FUNCTIONS IMAGE SOURCE This function is as described previously in Image Edit Screen - General Option. OTHER ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 106 Pan Up control Rotate Right control Reset Pan button Rotate Forward control Pan Down control Rotate Anti-clockwise control Pan Left control Reset Rotation button Pan Right control Rotate Clockwise control Rotate Backward control ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 107: Image Edit Screen - All Options Common Controls

     Selecting the View Images icon takes you back to the View Images screen, allowing you to view and compare captured images. The right hand group of Action icons is as follows: ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 108: Common Screen Functions And Tools

    The following general Action icons are available when using the Gel Doc software: The Home icon when selected takes you back to the Gel Doc Home screen. The Save icon is used to save captured/modified images. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 109: Saving Images

    From the pop-up save dialog box browse the file saving location and easily edit the file name before saving. All images will be saved in the .sgd file format which is a VWR data file which contains lots of useful diagnostic information particularly important if you have any support questions.
  • Page 110 Gel Doc image capture software will also automatically change the file name for the sub images too. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 111 From the pop-up window the list of images that you have selected will appear. Check the box to export all the images in the selected range and then ‘as displayed’ or ‘as captured’. Then press ok. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 112 – this can also be selected as a default setting in the setting menu. For publication purposes you can adjust the bit depth and dpi of tiff images Select ‘Save Selected’ button to save your images in the file format of your choice. ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 113: Printing

    The print function is actioned by selecting the Print icon from the bottom of the screen. What is printed also depends on the following:  Printing from the View Images screen.  Printing from the Image Edit screen. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 114 . The Printing series capture dialogue box is displayed. PRINTING SERIES CAPTURE DIALOGUE BOX Select the Display button. The additive image prints (blank sheet). OPTION 3 From View Images, to print a single constituent image: ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 115 Select one of the constituent image thumbnails. Select the Print icon . The Printing series capture dialogue box is displayed. PRINTING SERIES CAPTURE DIALOGUE BOX Select the Display button. The selected constituent image prints. MULTIPLEX CAPTURES OPTION 1 ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 116 From View Images, to print more than one constituent image: Select the constituent images. Select the Print icon The selected constituent images print. OPTION 5 From Image Edit, to print the multiplexed image plus the constituent images: ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 117 Select the All button. The multiplexed image plus all of the constituent images print. OPTION 8 From Image Edit, to print any one constituent image: Select one of the constituent image thumbnails. ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 118 Print function without printing by selecting the Report icon at the top left of the screen and selecting Close from the drop-down menu. EXIT WITHOUT PRINTING ® vwr.com Instruction manual VWR Imager Chemi Premium...

  • Page 119: Operator Maintenance

    Avoid spilling any liquid into the body of the instrument and clean any external spills immediately. If any liquid enters the main body of the instrument, make the instrument inoperative and contact the supplier. Contacting VWR International If it becomes necessary to contact VWR International the following information will be required:  Unit Serial Number ...

  • Page 120: User Replacable Accessories And Spare Parts

    • Complete technical service contact information • Access to the VWR Online Catalogue, and information about accessories and related products • Additional product information and special offers Contact us For information or technical assistance contact your local VWR representative or visit.

  • Page 121: Imager Chemi Premium Troubleshooting

    (2) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or refund the purchase price of this product to the customer, provided it is returned during the warranty period.

  • Page 122: Disposal

    By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health. Thank you ® vwr.com Instruction manual VWR Imager Chemi Premium...
  • Page 123 Local VWR offices in Europe and Asia Pacific Austria Hungary Poland International GmbH VWR International Kft. Graumanngasse 7 VWR International Sp. z o.o. Simon László u. 4. 1150 Vienna Limbowa 5 4034 Debrecen Tel.: +43 1 97 002 0 80-175 Gdansk Email: info.at@vwr.com...

This manual is also suitable for:

730-1469

Table of Contents