Table of Contents
Advertisement
5.
Check the box if you would like to image a visible marker.
6.
Select 'Auto Expose Area' if required.
7.
Press capture to capture an image. Note: If you have not pressed the capture button straight away
after the evaluation time then when you press capture a message will pop up 'Preview time has
expired' chemiluminescent signal may have decreased. Are you sure you would like to continue?'
Select 'Yes' to continue and capture the image. Select 'No' if you wish to stop capture and
reevaluate the capture time using the 'Eval. time' button.
8.
Press the 'stop' button to stop the capture at any time.
Chemi Blot (Series)
1.
Select your Blot type from the drop-down list e.g. Chemi Blot (Series).
2.
Select the reagent you are using (this is for reference only).
3.
Check the box if you would like to image a visible marker. Select the number of images you would
like to capture and the level of binning.
4.
Check the box to 'add the contents of the previous image'. This can help to improve the signal to
noise ratio providing smoother backgrounds.
5.
Press the 'Green arrow' to input the exposure time for each image or you can check the box to
'use the same exposure for all images'.
6.
On the sample positioning screen adjust the lens controls.
7.
Press the capture button to capture the image.
Signal Accumulation Calculator (S.A.C)
Signal Accumulation Calculator (S.A.C) allows the user to take a series of cumulative images. The User
Estimates the shortest and the longest exposure times required to achieve the optimal image. Then decide
on the number of images to be taken within this time range. For example select to capture 4 images and
specify shortest exposure time as 1 minute and the maximum as 4 minutes. The first image will be taken at
1 minute and the last at 4 minutes and the remaining two images are taken at even intervals in between at
1 minute each capture.
S.A.C. capture is useful for determining the optimal imaging time for a chemiluminescent sample. Note: For
very faint signals it is advisable to use Chemi Blot (Series) capture.
1.
Select your Blot type from the drop-down list e.g. Signal Accumulation Calculator.
30 vwr.com
Instruction manual
®
VWR
Advertisement
Table of Contents
