Operational Hints For The Vacuum Degasser - Agilent Technologies 1200 Series Reference Manual

Operational Hints for the Vacuum Degasser

Priming the Degasser
The vacuum degasser can be primed either by drawing solvent through the
degasser with a syringe or by pumping with the connected pump.
Priming the vacuum degasser with a syringe is recommended, when:
• vacuum degasser is used for the first time, or vacuum tubes are empty, or
• changing to solvents that are immiscible with the solvent currently in the
Priming the vacuum degasser by using the pump at high flow rate
(3–5 ml/min) is recommended, when:
• pumping system was turned off for a length of time (for example, overnight)
• if solvents have been changed.
When opening capillary or tube fittings solvents may leak out. Please observe
WA R N I N G
appropriate safety procedures (for example, goggles, safety gloves and protective
clothing) as described in the material handling and safety data sheet supplied by the
solvent vendor, especially when toxic or hazardous solvents are used.
Priming with a Syringe
Before using a new degasser or new tubings for the first time:
1 Prime all tubings with at least 30 ml of iso-propanol no matter whether the
If you are changing to a solvent that is immiscible with the solvent currently in
the tubing continue as follows:
2 Replace the current solvent with iso-propanol, if current solvent is organic
3 Disconnect solvent outlet tube of the channel that is supposed to be primed
4 Connect syringe adapter to solvent outlet tube.
5 Push syringe adapter onto syringe.
1200 Series VD Reference Manual
vacuum tubes.
and if volatile solvent mixtures are used, or
channels will be used with organic mobile phase or with water.
or with water, if current solvent is an inorganic buffer or contains salt.
from your pump.
Optimizing Performance
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