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USING REGIONS
CALIBRATION REGIONS
5.10 CALIBRATION REGIONS
If a region in a histogram contains a population of a known concentration, name the region
CAL to use it as a calibration region. There can be only one CAL region in each protocol or
panel. Do not enter the calibration factor in the region name.
To enter the calibration factor, for example, from the Flow-Count Fluorospheres package
insert:
1.
Setup Screen
2.
Statistics in the Functions box.
3.
CAL FACTOR in the Statistics box.
4.
Type the calibration factor found in the Flow-Count Fluorospheres package insert and
press Û.
The system multiplies the count and peak height statistics for all the regions by the
calibration factor, divided by the number of events in the calibrator region. The screen and
the printout display the adjustment factor. If the number of events in the calibrator region is
less than 1,000, the statistics are not adjusted.
The CAL Factor is a system function, not a protocol setting, and is not saved with the
protocol but applies to all protocols that have a Calibrator region.
Statistics adjusted by the CAL Factor are saved to the database record and can be printed on
patient reports.
CAL Factor adjustments to the statistics take precedence over hematology adjustments to the
statistics on patient reports.
The adjusted statistics can also be saved to the histogram file, to the export file, or to a
listmode file.
5-18
Protocol
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PN 4237238BA
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