Appendix B: Temperature Calibration Procedure; Introduction - Beckman Coulter Avanti J-26S XPI Instructions For Use Manual

Introduction

The Avanti J-26S XPI centrifuge specification for temperature control is 2°C of the set
temperature. That means that your sample will stay within 2°C of set temperature at all times, after
rotor and system equilibration, described below. (During transient conditions, such as acceleration
and deceleration, the rotor temperature may be outside this range.) The following procedure is
provided for those cases in which temperature control within 1°C is required.
In this procedure, you will measure the temperature of your sample after equilibrating the rotor
temperature for a minimum of 1 hour. Based on the test results, you can then adjust the centrifuge
temperature setting to bring your sample as close as possible to the required temperature.
1
For runs at other than ambient temperature, precool or prewarm the rotor and test samples
to the required temperature.
The length of precooling/prewarming time depends on how different the rotor starting
temperature is from the required run temperature.
2
Install the rotor with adapters, if applicable, and tubes or bottles filled with sample buffer or
water (if above 2°C).
3
Set the speed to 2000 RPM, the time to
temperature.
a. Start the run.
4
After 30 minutes, set the required run speed, and allow the system to run for at least 1 hour.
NOTE
The system must run for at least 1 hour before the temperature of the buffer or water is
measured. The length of time required for equilibration depends on the rotor and centrifuge starting
temperatures and the ambient room temperature.
5
After at least 1 hour, measure the temperature of the buffer or water using a thermometer or
thermocouple.
6
If the measured and set temperatures are different, note how many degrees different they are
and adjust the set temperature up or down that number of degrees.
PN B10093AE
Temperature Calibration Procedure
HOLD
, and the temperature to the required run
APPENDIX B
B-1