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Autogating works differently under certain circumstances:
Panel
Stop-on-volume
Autogated histogram You cannot autogate on a histogram if its gate equation has an
[PAUSE]
Assigning Autogating Regions
1.
Run the sample you want to assign an
autogating region to.
2.
Draw an amorphous region around the
population you want to autogate.
3.
Include AUTO, AUTO+, or AUTO++ in
the region name.
4.
Save the Protocol to save the region.
5.5
CELL-TRACKER REGIONS
Cell-Tracker software allows you to assign the events in a region to one of four colors. You
can use Cell-Tracker color analysis to identify populations without using gating. The color
assignments are stored with the protocol. Cell-Tracker software can be used for acquisition or
listmode replay.
PN 4237238BA
The primary and secondary protocols are used for Autogating; the
autogated region is passed on. You can edit that region in the
control protocol; the edited region is passed on.
Autogating occurs after data acquisition is complete.
autogated region in it.
Autogating does not occur whether in manual or automatic (MCL)
mode.
USING REGIONS
CELL-TRACKER REGIONS
5-11
5
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