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CREATING PROTOCOLS
DEFINING HISTOGRAMS
Histogram Resolution
Two-parameter histograms can be displayed in 64 x 64, 128 x 128, or 256 x 256 resolution.
Histogram resolution affects region statistics because region boundaries are different at
different histogram resolutions. The affected statistics can be normalized by toggling
[Normalize]on (green) in the Statistics pop-up window from the Protocol screen.
Positives Analysis
When you select the positives analysis (PosAnly) option, the software positions a positives
analysis region (linear or quad-stat) in the histogram at the end of the sample cycle.
If the histogram does not already have a region, the software creates one and names it
Positive.
If any regions already exist, but they are not positives analysis regions, the first region in a
histogram becomes a positives analysis region.
Positives analysis is typically used with the control histogram.
One-Parameter Histograms
For a one-parameter histogram (linear region), the software sets the:
Upper boundary of the region to the highest channel of the histogram.
r
Lower boundary of the region to the lowest channel needed to make the percentage of
r
events in the region approximate the percentage defined in the Functions box on the
protocol screen.
Autoscale to a multiple of four.
r
Two-Parameter Histograms
For a two-parameter histogram (quad-stat region), the software sets the region boundaries to
the lowest channels needed to make the sum of the percentage of events in:
Quadrants 1 + 2, not more than n%.
r
Quadrants 2 + 4, not more than n%.
r
You specify the value of n% in the Functions box on the Acquisition Protocol screen. In a
printout generated at the end of a sample cycle, the region's name is the only indication that
the region used positives analysis.
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PN 4237238BA
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