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CREATING PANELS
PANEL PROTOCOL ASSIGNMENTS
Changes made to the Cytometer settings in the other protocols in the panel are used and
saved only to those specific protocols (with [Save Cyto] turned on); they are not passed on.
The software can also pass the Cytometer settings from the QC data (*.QCS) file. For
protocols that have the same prefix used in the Auto Setup panels, for example 3CL
45-FITC/4-RD1/3-PC5, the Cytometer settings are accessed from the *.QCS data file
(3CL.QCS in this example) stored on the hard drive.
Passing Regions in a Panel
IMPORTANT
X-axis and Y-axis parameter assignments do not match, regions are not passed. If you want to pass the
regions in a panel, make sure that the parameters on the X- and Y-axes of a particular histogram number
match throughout a panel.
For example, the parameters on the X- and Y-axes of histogram 3 in the primary protocol
must match the parameters on the X- and Y-axes of histogram 3 in the other protocols in the
panel if the regions in histogram 3 are to pass from the primary protocol. If the parameters on
the axes do not match, the regions that existed in the new protocol are used; no passing into
that histogram occurs.
Passing Region Names in a Panel
Region names are passed from the primary protocol to the protocols that follow it.
Gating in a Panel
Histogram gate equations, which are used for gating, are not passed in a panel. If you want
the same gating used throughout a panel, give all the protocols in the panel identical gates
and gate equations, using the same region ID letters.
Cell-Seeker (Autogating) Regions in a Panel
In a panel, autogating works only in the primary and secondary protocols. The autogated
regions can be edited in the primary, secondary, and control protocols and passed on from
there.
Passing Other Regions in a Panel
Cell-Tracker (color assignment) equations, Calibration (CAL) regions, and Prism equations
are not passed in a panel.
6-4
Incorrect analysis results can be reported if regions are not passed in a panel. If histogram
PN 4237238BA
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