Labconco Logic+ 30348 Series User Manual page 45

Type b2 biosafety cabinets
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 Wash hands and arms thoroughly with germicidal soap
 Wear appropriate personnel protective equipment (PPE)
Wipe-Down
 Raise the sash to its full open position (approximately 21.75 inches or 552
mm). Mute the alarm by pressing
 Wipe down the interior surfaces of the cabinet with 70% ethanol, or a
suitable disinfectant, and allowed to dry
Loading Materials and Equipment
 Only load the materials required for the procedure. Do not overload the
cabinet
 Do not obstruct the front, side, or rear return air grilles
 Large objects should not be placed close together
 Slowly close the sash until it is in the correct operating position
 After loading the cabinet, wait two to three minutes to purge airborne
contaminants from the work area
Work Techniques
 Keep all materials at least 4 inches (100 mm) inside from the sash, and
perform all contaminated operations as far to the rear of the work area as
possible
 Segregate all clean and contaminated materials in the work area
 Arrange materials to minimize the movement of contaminated materials into
clean areas
 Keep all discarded contaminated material to the rear of the work area
 Avoid moving materials or the operator's hands and arms through the front
access opening during use
 Avoid the use of an open flame. Use disposable labware or an electric
incinerator as alternatives
 Use proper aseptic technique
 Avoid using techniques or procedures that disrupt the airflow patterns of the
cabinet
 If there is a spill or splatter during use, all objects in the cabinet should be
surface decontaminated before removal. Thoroughly disinfect the working
area of the cabinet WHILE IT IS STILL IN OPERATION, to prevent the
release of contaminants from the cabinet
5: Using The Cabinet
[OK/MUTE]
45

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