Table of Contents
Advertisement
Macro 900 Operation Manual
Be sure to handle chemicals with care and to read and comply with all
health and safety advice.
15.5.5.
Serial Dilution
The recommended Rhodamine solution is supplied as a 20% or 200g/L solution,
dilution of the stock solution should be carried out as follows.
200g/L stock → 100μg/L is recommended to be done as a two-step dilution
procedure.
Step 1; weigh out 0.5g of 200g/L stock solution in a weigh boat and add this to
1L of deionized water in a volumetric flask, use some of the water from the 1L
flask to rinse the weigh boat so no stock Rhodamine remains on the boat. Put
a lid on the 1L flask and invert 10 times.
This step results in a 1 in 2000 dilution of the stock, at this point the 1L flask
will contain a 100mg/L solution.
Step 2; Transfer 1ml of the 100mg/L solution to a 1L volumetric flask and top
up to 1L with deionized water. Put a lid on the 1L flask and invert 10 times.
This step results in a 1 in 1000 dilution of the solution from step 1.
concentration of this solution is 100μg/L. This solution can now be used as Pt-2
calibration of the RHOD sensor.
The dilute solution can be stored in a dark bottle in a refrigerator for up to five
days. After that time it must be discarded.
15.5.6.
Zero Point Calibration
To calibrate the zero point, follow these steps:
1. Fill a calibration bottle with distilled water, remove the storage cap from
the pH probe if fitted, wash the Probe in distilled water, then drop the
Probe in all the way. The Sleeve End Cap and Plug must be fitted.
Bang the Probe against the bottom of the bottle several times in order to
remove any air bubbles that may be clinging to the probe.
2. Switch the Macro 900 Meter on and wait until the temperature and Rhod
readings are stable. If the Rhod reading is very high, there are probably
air bubbles adhering to the lenses. Bang the Probe against the bottom of
the bottle to remove.
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF -
104ºF).
4. Press the MENU key then select Calibration. The following screen will be
displayed.
© 2013 Palintest Ltd.
Part number: 70301027
Description: Rhodamine WT Liquid
Supplier: Keystone Europe Ltd.
Contact: http://www.dyes.com
www.palintest.com
V3
The
Page 80 of 147
Advertisement
Table of Contents
Troubleshooting
