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Macro 900 Operation Manual
Differences in the fluorescent response between various types of oil.
Differences in the fluorescent response caused by temperature.
Differences in the fluorescent response caused by ambient light.
Interference caused by turbidity.
Fluorescence measurement techniques are ideal for researchers who are
interested in detecting the presence or absence of a specific substance and
measuring relative fluorescence changes that can be used as an indication of
increasing or decreasing concentrations.
Fluorescence
measurement
measurement. In order to obtain more accurate results, data obtained
with the fluorometer in the field should be post-calibrated with data
from standard laboratory analysis of grab samples acquired during the
study.
15.7.3.
Special Precautions When Using the REFOIL Probe
Always observe the safety advice printed above.
The UV LED is permanently on when power is supplied to the MAP
2000/2100 and draws 50mA. Switch the MAP 2000/2100 off between
readings to save power and prolong the UV LED's life.
Do not leave the MAP 2000/2100 powered on for prolonged periods if not
submerged. This can cause the UV LED to overheat.
Do not deploy the REFOIL probe in water temperatures above 30ºC.
Always carry out a zero point (Point 1) calibration as close to
operational temperature as possible prior to every use.
15.7.4.
Calibrating the REFOIL Probe
The REFOIL probe has two calibration points. Careful calibration is essential in
order to ensure consistent and reliable results. When a REFOIL probe is first
installed, it MUST be calibrated at both points in order to set the probe's
relative sensitivity and establish its slope.
Subsequently, a single point (Zero) calibration should be carried out daily.
Calibration of the REFOIL probe Zero point is normally carried out during
MacroCal (see
MacroCal Calibration
Full two-point calibration should be carried out every few months.
15.7.5.
Calibration Solution Preparation
In order to 'calibrate' the REFOIL probe, a 10ppm calibration solution of 1-5,
naphthalenedisulfonic acid disodium salt should be used. This solution contains
naphthalene, an aromatic hydrocarbon, which has similar fluorescence
characteristics to many Refined Oils.
The 10ppm calibration solution should be freshly prepared by serial dilution
from pure 1-5, naphthalenedisulfonic acid disodium salt. The following
Naphthalene salt is recommended:
© 2013 Palintest Ltd.
techniques
are
Method).
www.palintest.com
not
ideal
for
V3
quantitative
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