palintest Wagtech Potalab+ (M) Manual
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palintest Wagtech Potalab+ (M) Manual

Advanced portable water quality laboratory (microbiological)
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Wagtech
®
+ (M)
Potalab
®
Advanced Portable Water Quality
Laboratory (Microbiological)
Palintest
®
ZI PTW 10010M

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Summary of Contents for palintest Wagtech Potalab+ (M)

  • Page 1 Wagtech ® + (M) Potalab ® Advanced Portable Water Quality Laboratory (Microbiological) Palintest ® ZI PTW 10010M...
  • Page 2 Acquired by Palintest ® in 2011, the manufacture and support of the Wagtech ® portable water quality laboratory range has now been integrated into the Palintest ® product family. ® Further information regarding the Wagtech product range can be found at: www.palintest.com...

  • Page 3: Table Of Contents

    Contents Chapter Page Kit Layout Introduction Preparation Taking a Sample Sample Processing - Membrane Filtration Incubation and Incubator Operation Microbiological Results Appendix 1 - Field Testing Checklist - Hints Appendix 2 - Troubleshooting Appendix 3 - Technical Specifications Appendix 4 - Reagents and Consumables...

  • Page 4: Kit Layout

    Kit Layout Potalab ® + (M) Water Test Kit - Layout Fig 1. Potalab ® + (M) Water test kit opened to show contents. Coloured circles indicate the chapter colour in which their use is explained.
  • Page 5 Kit Layout Function Equipment Preparing the Membrane Lauryl Sulphate Broth (MLSB) Media/Petri Dishes Media Measuring Device (MMD) Absorbent Pads Pad Dispenser Petri Dishes (in incubator) Membrane Filtration Membrane Filtration Unit Hand Vacuum Pump Forceps 47mm Membrane Filters Incubation and Incubator Incubator Operation Mains Power Supply Battery...

  • Page 6: Introduction

    This instruction manual is also available in French, Spanish and Mandarin. Additional advice and training is available upon request. Contact us directly at support@palintest.com or via your local representative. 2.1 Before You Use Your Kit Thermotolerant Coliforms (sometimes called Faecal Coliforms).
  • Page 7 Introduction Total Coliforms refers to a large group of Gram- Membrane Filtration Method negative, rod-shaped bacteria that share several characteristics. The group includes Thermotolerant Coliforms and bacteria of faecal origin, as well Water sample - filtered as some bacteria that may be isolated from through a membrane filter environmental sources.
  • Page 8 Introduction A vacuum hand pump attached to the MFU Useful to Know creates suction that pulls the sample water through a sterile membrane filter that sits in • Always wash your hands before place in the MFU. carrying out your microbiological analysis, and again after handling This membrane filter has small pores in it that potentially contaminated materials...

  • Page 9: Preparation

    Preparation 3.0 Preparation Every effort should be made to keep the kit and all its components clean and free from contamination. At all times you should work in a manner that limits the chance of cross contamination of your samples. 3.1 Aseptic Procedure Useful to Know There are specific techniques and methods that...
  • Page 10 Preparation 3.2 Sterilisation of the for sterilisation (see below), which will leave Membrane Filtration Unit a small gap between the bottom of the filter funnel and the silicone base. Upon receipt of your water test kit the Membrane Filtration Unit (MFU) must be washed thoroughly and then dried with a clean cloth or paper towel.
  • Page 11 Preparation 3.3 Sterilisation of the 3 Remove the lid Aluminium Petri Dishes of the petri dish and dispense a Your Wagtech test kit is supplied as standard with pad into the dish a set of aluminium petri dishes. These are used by sliding back during the microbiological analysis.
  • Page 12 Preparation 3.6 Preparing Culture Media in the Field 7 Once cool, unscrew the blue lid/spoon of the using the Media Measuring Device (MMD) MMD. Take ten level spoonfuls of media from the 38.1g container of the MLSB and add to the Items Required: MMD.
  • Page 13 Preparation 3.7 Tyndallisation of MMDs 3.8 Storage of Liquid Media In the event an autoclave or pressure cooker is The sterile MLSB should be stable for up to not available then the sterilisation of the MMD 6 months if stored in a refrigerator. If no containing the liquid MLSB media can be carried refrigerator is available the media can be stored out using a pan of boiling water.
  • Page 14 Preparation Useful to Know • Growth or culture media is a substance designed to support the growth of microorganisms (bacteria). The media is a vital part of the microbiological water quality test • There are different types of media for growing different types of bacteria.

  • Page 15: Taking A Sample

    Taking a Sample 4.0 Taking A Sample The optimum volume of sample is that which will allow the most accurate enumeration of bacteria. The technique of membrane filtration is unsuitable for natural waters containing very high levels of suspended material, sludges and sediments, all of which could block the filter before an adequate volume of water has been filtered.

  • Page 16: Sample Processing - Membrane Filtration

    Sample Processing - Membrane Filtration 5.0 Membrane Filtration of the Sample Items Required: • Membrane Filtration Unit • MMD containing Liquid MLSB Media • Pistol Grip Hand Vacuum Pump • Petri Dishes containing Absorbent Pad • Forceps/Tweezers • Methanol (not supplied) •...
  • Page 17 Sample Processing - Membrane Filtration 6 Lock the membrane filter in place by pushing 9 Discard the excess water from the sampling cup the filter funnel firmly into position in the blue then insert the filter funnel/base assembly rubber base. Take care to not touch the internal into position in the sampling cup.
  • Page 18 Sample Processing - Membrane Filtration 12 When all of the sample has been filtered, 16 Lift the petri dish containing the absorbent pad detach the vacuum pump and remove the and hold between thumb and forefinger. filter funnel from the rubber base. The membrane filter is now ready to be removed and placed in the petri dish containing the absorbent pad and growth media.
  • Page 19 Sample Processing - Membrane Filtration 19 Use the sterile forceps to remove the Environmental exposure can cause coliforms to membrane filter from the filtration unit. become physiologically stressed. The resuscitation period allows coliforms to recover before culturing. With this in mind it is essential to plan testing throughout the day, particularly if visiting multiple sample sites.

  • Page 20: Incubation And Incubator Operation

    Incubation and Incubator Operation 6.0 Operation of Incubator The Potalab ® + incubator is a high performance field incubator designed to deliver reliable Total and Thermotolerant Coliform results in even the most extreme circumstances. Providing at least 5 cycles of incubation under standard conditions when battery powered, the incubator is simple to operate and provides performance data throughout the incubation cycle.
  • Page 21 Incubation and Incubator Operation 6.2 Recharging the 12V DC Battery • A red LED in the right hand corner of the incubator lid indicates that power is supplied Note: For optimal recharging, switch off and the incubator is on. The backlight will the incubator.
  • Page 22 Incubation and Incubator Operation User ID The Potalab ® + incubator also includes optional profiles for up to 6 Custom Profiles. Select or edit the username using alphanumeric characters. There are 8 separate User ID profiles. To set a Custom Test profile: •...
  • Page 23 Incubation and Incubator Operation Set Date To set the Date: • Highlight ‘Set Date’ and press ‘OK’ • Use the UP/DOWN keys to set the day. When correct press ‘OK’ • Use the UP/DOWN keys to set the month. When correct press ‘OK’ The Check Calibration function provides a simple •...
  • Page 24 Incubation and Incubator Operation 6.3.3 Incubation Menu There are five standard prompt cards with corresponding audio, both indexed by letter The ‘Incubation’ menu is accessed from and number. the ‘MODE’ screen, confirms the incubator settings and starts the incubation cycle. The Prompt Cards are grouped as follows: •...
  • Page 25 Incubation and Incubator Operation 6.4 Incubating Samples • The incubator has a default warm-up period of 30 minutes at the start of the incubation The incubation of samples can begin once the process. During this phase the incubator will correct resuscitation period has been observed display ‘warming up’...
  • Page 26 Pota Pal app. To update firmware: • Connect the incubator to the device using the supplied USB cable and wait for installation •...
  • Page 27 Incubation and Incubator Operation • Select ‘View Incubation Data’ • Select the Prompt Card required for the audible instructions (see section • The dialogue screen will list the incubation 6.3.4 and existing prompt cards) cycles available for view listed by date/time, incubation protocol and number •...
  • Page 28 The internal validation of thermistor temperature For service please contact your local monitoring has exceeded the tolerance of the Palintest representative or email to device. Return to authorised service centre for support@palintest.com. attention as soon as possible. Error 110: Battery Low The 12V DC battery has insufficient battery capacity to complete the full incubation cycle.

  • Page 29: Microbiological Results

    Microbiological Results 7.0 Microbiological Results Bacteria in water are generally not present individually but as clumps or in association with particulate matter. When enumerating bacteria in water it is not the number of individual bacteria present which are counted but the number of clumps of bacteria or the particles and their associated bacteria. Each clump or particle may have many bacteria associated with it.

  • Page 30: Appendix 1 - Field Testing Checklist - Hints

    Appendix 1 - Field Testing Check List - Hints Note: Field testing requires careful planning Ensure you have considered the following When you are in the field: additional items that may be required in • Find a flat area! Always place kit on a addition to the Potalab ®...

  • Page 31: Appendix 2 - Troubleshooting

    If the capacity is less Should any condition arise requiring maintenance than the power required the warning will or service, please contact your local Palintest be displayed. Select ‘Accept’ to continue with representative or via support@palintest.com the incubation cycle but find an additional The Potalab ®...

  • Page 32: Appendix 3 - Technical Specifications

    The Potalab + incubator is designed for incubation of microbiological samples in petri dishes. ® Follow the instructions regarding connection of power cables carefully and only use genuine Palintest cables/chargers. Using the Potalab ® + incubator in a manner not specified by Palintest may affect...

  • Page 33: Appendix 4 - Reagents And Consumables

    Appendix 4 - Reagents and Consumables Membrane Filtration Consumables Part Number Description PTW 10452 Membrane Lauryl Sulphate Broth, 500g PTW 10454 Membrane Lauryl Sulphate Broth, 38.1g PTW 10456 1.92g Membrane Lauryl Sulphate Broth, pack of 25 PTW 10459 Membrane Filters, 47mm diameter, 200 pack PTW 10460 Absorbent Pads and Membranes, 200 pack PTW 10461...
  • Page 34 Palintest Ltd Palintest House, Kingsway, Team Valley, Gateshead, NE11 0NS Tel: +44 (0)191 491 0808 Fax: +44 (0)191 482 5372 sales@palintest.com (UK) export@palintest.com (International) Palintest Australia and Asia Pacific 1/53 Lorraine Street, Peakhurst Business Centre, Peakhurst, NSW 2210, Australia Tel: +61 1300 131516 Fax: +61 1300 131986 palintest@palintest.com.au...

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