Principle Of Operation; Pt 1555 Fluorescein Dye Probe; Limitations Of Use; Calibrating The Fscein Probe - palintest Macro 900 Operation Manual

Macro 900 Operation Manual

15.6. PT 1555 Fluorescein Dye Probe

Fluorescein is a fluorescent dye that is commonly used in water flow studies
and can be measured by the MAP 2000/2100 using the optional PT 1555
Fluorescein Dye optical probe.
15.6.1.

Principle of Operation

The PT 1555 Fluorescein Dye optical probe is a submersible, fixed response
fluorometer, which provides excitation at 470nm and detects any resultant
fluorescence above 550nm.
The probe induces the Fluorescein to fluoresce, then measures the longer
wavelength light which is emitted as a result of the fluorescence process.
15.6.2.

Limitations of Use

Measurement of Fluorescein in the field using fluorescence measurement
techniques can be adversely affected by:
Interference
fluoresce at similar wavelengths.
Differences in the fluorescent response caused by temperature.
Differences in the fluorescent response caused by ambient light.
Interference caused by turbidity.
The normal effects of temperature on the fluorescent response of Fluorescein is
automatically compensated for by the probe.
15.6.3.

Calibrating the FSCEIN Probe

The FSCEIN probe has two calibration points. Careful calibration is essential in
order to ensure consistent and reliable results.
When a FSCEIN probe is first installed, it MUST be calibrated at both points
in order to set the probe's relative sensitivity and establish its slope.
Subsequently, a single point (Zero) calibration should be carried out daily.
Calibration of the FSCEIN probe Zero point is normally carried out during
MacroCal (see MacroCal Calibration
Full two-point calibration should be carried out every few months.
15.6.4.

Calibration Solution Preparation

In order to 'calibrate' the FSCEIN probe, a 100µg/L calibration solution of
Fluorescein Dye should be used.
The 100µg/L calibration solution should be freshly prepared by serial dilution
from 200g/L standard using deionised water. The following Fluorescein Dye is
recommended:
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