Table of Contents
Advertisement
Macro 900 Operation Manual
Be sure to handle chemicals with care and to read and comply with all
health and safety advice.
15.6.5.
Serial Dilution
A three step dilution process should be used as outlined below.
Step 1; Weigh out 0.5g Fluorescein dye powder and add to 1L deionized water
in a volumetric flask.
gives a stock solution of 500mg/L.
Step 2; Transfer 10ml of the 500mg/L stock solution into a 1L volumetric flask
and top the flask up to 1L with deionized water. Invert to mix.
This step results in a 1 in 100 dilution of the 500mg/L stock resulting in a
5mg/L stock.
Step 3; Transfer 20ml of the 5mg/L stock from step 2 into a 1L volumetric
flask. Top up to 1L with deionized water. Invert to mix.
This step results in a 1 in 50 dilution and gives you the 100μg/L FSCEIN
calibration standard required for Pt-2.
The dilute solution can be stored in a dark bottle in a refrigerator for up to five
days. After that time it must be discarded.
15.6.6.
Zero Point Calibration
To calibrate the zero point, follow these steps:
1. Fill a calibration bottle with distilled water, remove the storage cap from
the pH probe if fitted, wash the Probe in distilled water, then drop the
Probe in all the way. The Sleeve End Cap and Plug must be fitted.
Bang the Probe against the bottom of the bottle several times in order to
remove any air bubbles that may be clinging to the probe.
2. Switch the Macro 900 Meter on and wait until the temperature and Fcein
readings are stable. If the Fcein reading is very high, there are probably
air bubbles adhering to the lenses. Bang the Probe against the bottom of
the bottle to remove.
3. Ensure the temperature of the solution is between 5ºC and 40ºC (41ºF -
104ºF).
4. Press the MENU key then select Calibration. The following screen will be
displayed.
© 2013 Palintest Ltd.
Part number: 801 073 81
Description: Keyacid Fluorescein 019187
Supplier: Keystone Europe Ltd.
Contact: http://www.dyes.com
Invert 10 times or until all powder is dissolved.
www.palintest.com
V3
This
Page 84 of 147
Advertisement
Table of Contents
Troubleshooting
