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Macro 900 Operation Manual
15.5. PT 1554 Rhodamine WT Probe
Rhodamine WT is a fluorescent red dye that is commonly used in water flow
studies and can be measured by the MAP 2000/2100 using the optional PT
1554 Rhodamine WT optical probe.
15.5.1.
Principle of Operation
The optional PT 1554 Rhodamine WT optical probe is a submersible, fixed
response fluorometer, which provides excitation at 520nm and detects any
resultant fluorescence above 575nm.
The probe induces the Rhodamine to fluoresce, then measures the longer
wavelength light which is emitted as a result of the fluorescence process.
15.5.2.
Limitations of Use
Measurement of Rhodamine in the field using fluorescence measurement
techniques can be adversely affected by:
Interference
fluoresce at similar wavelengths.
Differences in the fluorescent response caused by temperature.
Differences in the fluorescent response caused by ambient light.
Interference caused by turbidity.
The normal effects of temperature on the fluorescent response of Rhodamine is
automatically compensated for by the probe.
15.5.3.
Calibrating the RHOD Probe
The RHOD probe has two calibration points. Careful calibration is essential in
order to ensure consistent and reliable results.
When a RHOD probe is first installed, it MUST be calibrated at both points
in order to set the probe's relative sensitivity and establish its slope.
Subsequently, a single point (Zero) calibration should be carried out daily.
Calibration of the RHOD probe Zero point is normally carried out during
MacroCal (see
MacroCal Calibration
Full two-point calibration should be carried out every few months.
15.5.4.
Calibration Solution Preparation
In order to 'calibrate' the RHOD probe, a 100µg/L calibration solution of
Rhodamine WT should be used. This is exactly the same calibration solution
that is recommended for calibration of the BGA-PC probe.
The 100µg/L calibration solution should be freshly prepared by serial dilution
from 200g/L standard using deionised water. The following Rhodamine WT
standard is recommended:
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