Developing Methods; Measuring Dwell Volume - Waters ACQUITY Arc System System Manual

2.5.1.10 High-pressure pin plug
First use or re-installed
Tighten the pin plug finger-tight, plus up to 1/6-turn using a wrench.

2.6 Developing methods

See also:
For information about method development and validation, consult the
Auto•Blend Plus Technology for Ion Exchange, Size Exclusion, and Reversed-phase
Chromatography documentation.

2.7 Measuring dwell volume

When transferring a gradient LC method, you must calculate the dwell volume of the
two systems. The dwell volume is the system volume between the point where the
gradient is formed and the column inlet. The dwell volume varies from system to
system but is typically between 0.1 and 2.0 mL.
You can measure dwell volume using the midpoint of a 0–100% gradient. To do so,
run a gradient between two identical solvents, A and B, spiking the B solvent spiked
with a marker. Perform the measurement after configuring the system without the
column for the method being transferred, replacing the column with a low-volume
restrictor, to ensure proper pump function.
See also:
"Measuring system volume for methods transfer" in the console online
Help.
June 12, 2015, 715004747 Rev. A
Page 30