tecan M1000 Operating Manual Addendum page 28

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13 At the Load DiTis prompt, select Using Full DiTi Racks. Add full 1000 uL DiTi
racks to Plate Carrier 1, Loc 1 and 2, and Plate Carrier 2, Loc 1 and 2. Add a
full 200 uL DiTi rack to Plate Carrier 2, Loc 3. Add a full 1000 uL DiTi rack to
Plate Carrier 1, Loc 3, the DiTi Reuse Rack.
14 At the checklists, select > to continue.
15 Select <Scan Reagents>.
16 Enter a value for the Reagent Lot ID field and select > to continue.
17 Select <Flush System>. Select > to continue.
18 At the Acknowledge screen, select > to continue.
19 The operator is prompted "Use Full DiTi Boxes only". Select <OK>.
20 The operator is prompted "Plate Carrier 1 Loc 1, 1000 DiTi". Select <OK>.
21 Observe the LiHa as it picks up the disposable tips. Determine if any
adjustment is needed for the DiTi cone to be centered over the DiTis and note
the required adjustment.
Note: The adjustment will be made in Step 33.
22 The Check DiTi Locations protocol will access each of the 6 locations,
prompting the user prior to moving to the next Plate Carrier location. To
proceed with the next location, select <OK>.
23 When the Check DiTi Locations protocol completes, select <Finish>. Enter
your User Name and password and select > to proceed to the next screen.
24 Exit NAT Wizard and select <Exit>.
25 Select <Yes>.
26 Open NAT Admin.
27 Enter your User Name and Password and <OK> to proceed.
28 Select File from the NAT Admin main screen.
29 Select Teach Worktable Locations.
30 Select Connect to the Instrument.
31 Select Initialise all Devices.
Note: This step is only needed if the instrument has NOT been initialized. It is not
necessary to initialize all devices each time you enter NAT Admin unless there
has been an error with a device.
32 Remove the DiTi rack carriers.
33 Select Calibrate LiHa. Select a carrier from the list box icon located under the
Current Rack field. The following positions on the worktable need to be
adjusted for X and Y direction based upon the observations from the Check
DiTi Locations protocol:
– Plate Carrier 1, Loc 1
– Plate Carrier 1, Loc 2
– Plate Carrier 1, Loc 3
– Plate Carrier 2, Loc 1
– Plate Carrier 2, Loc 2
– Plate Carrier 2, Loc 3
Note: Only adjust by 5, 10, 15, or 20 steps. DO NOT adjust the Z direction. Once
each location is adjusted, select Save LiHa Calibration.
34 Raise the Z direction for only Plate Carrier 1, Loc 3 (DiTi Reuse Rack)
28 steps. Select Save LiHa Calibration.
m1000™ Operating Manual Addendum, 392813, en, V1.1