Temperature Check - tecan M1000 Operating Manual Addendum

Temperature
Check
m1000™ Operating Manual Addendum, 392813, en, V1.1
The temperature check for the 1 ml subsystem should be performed according to
your laboratory procedures or as directed for troubleshooting. Refer to the
m1000™ Operating Manual, Chapter 8, Troubleshooting, for additional
assistance.
WARNING
Potentially infectious
Instrument parts may be contaminated with potentially infectious materials.
Follow basic biohazard precautions
Wear appropriate personal protective equipment, such as gloves, lab coats
and protective eye wear
WARNING
Hot Surface Hazard: Identifies an area where the operator may be exposed to hot
surfaces, such as Heater Zone 1 and Heater Zone 2.
Perform the following steps:
1 Power on the m1000™, if necessary.
2 Select the NAT Wizard icon from the desktop screen. The Main menu is
displayed.
3 From the Main Menu, select Select Protocol.
4 The Authorization dialog window is displayed. Enter your User Name and
password and select > to proceed to the next screen.
5 Select the Abbott 1 ml Protocol v1.0 or the Generic 1 ml Protocol v1.0 to be
run.
6 Place one reaction vessel in the 1 ml subsystem carrier (any location). Add
approximately 1 ml of water, or enough to cover the base of the thermometer.
Place the 1 ml subsystem carrier into heater zone 1.
7 Select > to proceed.
8 The operator is prompted to initiate the Pre-heat protocol to allow heater
zone 1 to reach correct temperature. Select <OK>.
9 Once heater zone 1 reaches the correct operating temperature, the operator
is prompted Heater Zone 1 Achieved Operating Temperature. Select
<OK>.
10 Insert a thermometer into the reaction vessel in heater zone 1. Allow to
equilibrate to 50°C (± 2.5°C). Verify this temperature is 50°C (± 2.5°C).
Note: DO NOT place the thermometer into the reaction vessel until the Scan
Rack screen displays.
11 Move the 1 ml subsystem carrier to heater zone 2. Allow the thermometer to
equilibrate to 75°C (± 2.5°C). Verify this temperature is 75°C (± 2.5°C).
12 If the temperature for heater zone 1 and heater zone 2 are not within the
specified range, repeat this procedure. Do not run a sample preparation
protocol until the temperature for both heater zones are correct. Refer to the
m1000™ Operating Manual, Chapter 8, Troubleshooting, for additional
assistance.
13 Remove the thermometer.
14 Select < twice to return to the Main Menu.
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