3
Good Measurement Practices
General Considerations
Figure 9
Lens tissues for glasses or other uses often contain detergents or lubricants
which can affect your measurements. If possible avoid cleaning the faces of
your cell between blank and sample measurements.
Handling Cells
Always install a cell so that it faces the same direction to minimize problems
with cell non-uniformity. For best results with microcuvettes, leave your
sample cell clamped in position throughout the measurement sequence.
Solutions should be removed and replaced by pipette or use flow cells.
If glass pasteur pipettes are used, make sure that the optical windows of the cell are
CAUTION
not touched or scratched by the pipette.
38
0.01
0.00
-0.01
-0.02
-0.03
-0.04
-0.05
200
400
Spectrum taken with floating particles in the light path
Agilent Cary 8454 UV-Visible Spectroscopy System Installation Guide
600
800
Wavelength [nm]
1000