System Overview - GE ReadyToProcess WAVE 25 Operating Instructions Manual

3 System description

3.1 System overview

3.1

System overview

Introduction
ReadyToProcess WAVE 25 is intended for cell cultivation.
A disposable Cellbag bioreactor is placed on a rocker and filled with gas, partially filled
with culture medium, and inoculated with cells. Gas transfer and mixing of culture is
accomplished by wave-induced agitation, performed by the rocker unit.
The cell culture volume range per Cellbag bioreactor is 0.3 to 25 L depending on biore-
actor size, and working volume may be expanded up to 10 times during one cultivation.
The system, composed of the rocker, ReadyToProcess CBCU and ReadyToProcess Pump
25, enables measurement and control of pH, DO, weight and media distribution, and
provides different gas flow and gas mixing possibilities.
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The system is controlled from a PC running UNICORN software version 7 or later. The
system can also be controlled from a supervisory control and data acquisition (SCADA)
system, such as the Emerson DeltaV™ control system, using the integrated OPC server.
Contact GE for instructions and guidance for OPC.
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In single mode, the system supports culture in one Cellbag bioreactor at one time.
The rocker is connected to one ReadyToProcess CBCU and up to three
ReadyToProcess Pump 25 units.
In dual mode, the system supports culture in two Cellbag bioreactors placed on the
same tray. The rocker is connected to up to two ReadyToProcess CBCU units and up
to three ReadyToProcess Pump 25 units for independent control of culture conditions
in the two bioreactors.
ReadyToProcess WAVE 25 Operating Instructions 29009597 AD