System Overview - GE ÄKTA start Maintenance Manual

System overview

Part Description
1 ÄKTA start
 Frac30, Fraction collector
3 PC with UNICORN™ start software
Main features of ÄKTA start
• ÄKTA start is operated in two modes: 1) using Instrument
Display (touch screen), and 2) using UNICORN start.
• ÄKTA start is offered with a dedicated Fraction collector,
Frac30.
• Quick start methods available for easy purification of
common tagged/untagged proteins.
• Method templates available for Affinity Chromatography
(AC), Ion Exchange Chromatography (IEX), Gel Filtration
(GF) and Desalting (DS).
• Predefined methods available for cleaning the system
flow path, flow cells (UV and Conductivity) and testing the
system performance.
Instrument Display - home screen
The ÄKTA start home screen displays four options for the
user to select and perform operations from.
Method run: run predefined methods
Manual run: start and control the system in real time

3
Create method: create, edit, import and delete methods
Settings and service: configure, calibrate, troubleshoot
and diagnose modules
Instrument illustration
The illustration below shows the locations of the modules
placed on the wet side of the instrument. The modules
have the following functions:
• To deliver the liquid in the system flow path and divert
the flow as required
• To monitor the UV absorbance and conductivity of the
liquid
1
2
3
Part
1

3
4
5
1
8
4 5
Description Part
Buffer valve 6
Mixer 7
Sample valve 8
Pump 9
Pressure sensor 10
ÄKTA start Maintenance Cue Card, 29-0240-43 AC
2
9 10
7
6
Description
Wash valve
Injection valve
UV Monitor
Conductivity Monitor
Outlet valve