Using The Gradient Calculator - BIO RAD PTC-200 Operation Manual

Using the Gradient Calculator

The gradient calculator predicts, for a given gradient, the temperatures for each of
the twelve wells along the long-axis of the sample block. These temperatures will be
predicted from an algorithm as a steady-state temperature of the block and the
sample. To use the gradient calculator, select the List command from the Main Menu
and then press the «Proceed» key. You will see a List screen:
Select Gradient Calculator and press the «Proceed» key :
Enter lower limit temperature of the gradient using the number keys and then press
the «Proceed» key. Enter the upper temperature for the gradient using the number
keys and «Proceed» key. You may enter integers without decimal points. The following
screen will be displayed:
Gradient Calculator:
Column 1- 50.0 left
Column 2- 50.5
Column 3- 51.5
Column 4- 53.2
Column 5- 55.5
Column 6- 58.4
Column 7- 61.8
Column 8- 64.6
Column 9- 66.8
Column 10- 68.4
Column 11 - 6 9 . 6
Column 12- 70.0 right
You may scroll up and down the screen using the «Select» keys. When scrolling, the
"Gradient Calculator" line will be held constant while the lower 3 lines can be manipulated
up or down.
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
L i s t :
Programs
_GRADIENT CALCULATO R
Lower Temp C : 50.0
Upper Temp C : 70.0
Creating Programs
6-11