Modifying Block-Control Programs For Calculated Control; Modifying A Program Designed For A Different Machine; Entering A New Program; Initiating The Program - BIO RAD PTC-200 Operation Manual

Modifying Block-Control Programs for Calculated Control

Block-control programs can be changed to calculated control by subtracting at least
15–20 seconds from each temperature step. Some empirical testing may be required to
adjust modified programs for optimum performance.

Modifying a Program Designed for a Different Machine

The ramp programming step can be used to adapt programs designed for thermal
cyclers with slower maximum heating and cooling rates than the DNA Engine cycler. In
addition, a given protocol will occasionally work better with a slower rate of temperature
change; the ramp step can be used to optimize the program for such a protocol.

Entering a New Program

Programming the DNA Engine cycler moves through five steps:
1.
2.
3.
4.
5.
Each step involves entering values from the keyboard or making selections from a menu.
Programs may be edited as they are being entered.
Programs are automatically saved when the End step is entered. They are stored in the
<MAIN> folder unless folders have been created for them.

Initiating the Program

To initiate a new program, select Enter from the Main Menu, then press «Proceed». A
naming screen will be displayed:
Initiating the program
Naming the program
Choosing a temperature control method
Entering the program's steps
Entering the End step
Enter:
Name: A
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Creating Programs
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