Editing A Gradient Step; Reviewing A Gradient Program - BIO RAD PTC-200 Operation Manual

DNA Engine Operations Manual

Editing a Gradient Step

To edit a gradient step, select Edit from the Main Menu. The program will be displayed
as follows:
Use the «Select» keys to scroll to the step you want to edit.

Reviewing a Gradient Program

After the protocol has been run, the Program Complete screen will be displayed. Press the
«Proceed» key, and the next screen will display a review of the gradient well temperatures:
Gradient Review:
Column 1- 50.0 left
Column 2- 50.5
Column 3- 51.5
Column 4- 53.2
Column 5- 55.5
Column 6- 58.4
Column 7- 61.8
Column 8- 64.6
Column 9- 66.8
Column 10- 68.4
Column 11 - 6 9 . 6
Column 12- 70.0 right
Scroll up and down the screen using the «Select» keys. When scrolling the "Gradient
Review" line will be held constant while the lower 3 lines can be manipulated up or down.
The temperatures displayed will be for each of the twelve wells along the long-axis of the
sample block. These temperatures will be predicted from an algorithm as a steady-state
temperature of the block and the sample. You may then return to the Main Menu by
pressing the «Proceed» key.
6-10 Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
List: CUSTO M 1
1= 92.0 f o r 0 : 2 0
2= 50.0 t o 70.0
f o r 0 : 3 0