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PTC-0240
BIO RAD PTC-0240 Manuals
Manuals and User Guides for BIO RAD PTC-0240. We have
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BIO RAD PTC-0240 manual available for free PDF download: Operation Manual
BIO RAD PTC-0240 Operation Manual (148 pages)
DNA Engine. Peltier Thermal Cycler
Brand:
BIO RAD
| Category:
Laboratory Equipment
| Size: 1 MB
Table of Contents
Table of Contents
4
Explanation of Symbols
5
Safety Warnings and Guidelines
5
Documentation Conventions
7
Chapter 1: Introduction
8
Layout and 2
12
DNA Engine Tetrad 2 Cycler Control Panel
13
DNA Engine Tetrad 2 Cycler Back View
14
DNA Engine Tetrad 2 Cycler Bottom View
14
Compatible Alpha Units
15
DNA Engine Tetrad 2 Specifications
16
Gradient Specifications (96-Well Alpha Unit Only)
16
3 Installation
18
Packing Checklist
19
Setting up the DNA Engine Tetrad 2 Cycler
19
Unpacking and Moving the DNA Engine Tetrad 2 Cycler
19
Environmental Requirements
20
External Mouse Device
20
Air Supply Requirements
21
Ensuring an Adequate Air Supply
21
Ensuring that Air Is Cool Enough
22
Power Supply Requirements
21
Requirements for Robotics Installations
22
384-Well Microplate Specifics
23
4 Operation
24
Turning the DNA Engine Tetrad 2 Cycler on
25
Chapter 2 : Layout and Specifications
26
Chapter 3 : Installation
26
Chapter 4: Operation
26
Using the Control Panel
26
Display Screen
26
Operation Keys
26
Block Status Lights
27
Using the Data Ports
27
Operating Alpha Units
27
Installing an Alpha Unit
27
Removing an Alpha Unit
28
Closing an Alpha Unit
29
Opening an Alpha Unit
29
Selecting the Correct Sample Vessel
29
0.5 Ml Tubes
30
Microplates
30
Thin-Walled Vs. Thick-Walled Tubes
30
Sealing Sample Vessels
31
Sealing with Oil or Wax
31
Sealing with the Hot Bonnet Lid
31
Adjusting the Hot Bonnet Lid's Pressure
32
Loading Sample Vessels into the Block
33
Using Oil to Thermally Couple Sample Vessels to the Block
33
Appendix 4-A: Tube, Microplate, and Sealing System Selection Chart
35
Appendix 4-B Safety Warning Regarding Use of S Nucleotides
36
5 Creating Programs
38
Front Panel Setup
40
Display Screen
40
Navigation Keys
40
Numeric Keypad
41
Programming Conventions
41
The Elements of a Program
41
Types of Programs
43
Graphical Programs
43
Advanced Programs
44
Designing a New Program
44
Let's Start with an Example
45
The Goto Option
45
Considerations During Program Creation
46
Choosing a Temperature Control Mode
46
Calculated Control
46
Block Control
47
Modifying a Program Designed for a Different Machine
47
Modifying Block-Control Programs for Calculated Control
47
Choosing a Lid Control Mode
48
Choosing a Temperature Ramping Rate
48
Choosing a Temperature Hold Time
48
Choosing a Thermal Gradient
49
Beyond the Example Protocol: Other Considerations
50
Entering Program Steps
51
The Status Window
51
Entering a Program Using Graphical Mode
52
Using the Mode Selection Window
53
Using the File Save as Window
54
Folder Passwords
56
Editing Step Parameters
57
Selecting a Step
57
The Graphical Programming Window
57
Adding a Step
58
Deleting a Step
58
Entering a Temperature Step
58
Entering a Gradient Step
61
Entering a Goto Step
62
Entering a Forever Incubation
63
Entering a Program Using Advanced Mode
64
Entering a Temperature Step
65
Entering a Gradient Step
68
The Extend Time Option
70
Entering a Goto Step
71
Entering a Lid Control Step
72
The Slow Ramp Option
72
The Increment Temp Option
73
Managing and Editing 6
76
Opening a Program
77
Opening a Program in Advanced Mode
77
Chapter 5 : Creating Programs
78
Chapter 6: Managing and Editing Programs
78
Opening a Program in Basic Mode
79
Editing a Program
79
Editing a Graphical Program
80
Deleting a Step
81
Editing an Advanced Program
81
Highlighting and Selecting Program Steps
81
Editing a Step
82
Inserting a Step
82
File Utilities
83
Saving an Edited Program
83
Copying a Program
84
Deleting a Program
85
Deleting a Folder
86
Moving a Program
86
7 Running Programs
88
Using the Instant Incubation Feature
89
Chapter 7: Running Programs
90
Running Programs
91
The Run Program Window
92
During the Run
93
Run Status
93
Pausing/Resuming a Run
95
Skipping a Step
95
Terminating a Run
95
Inaccessible Features
95
Running Multiple Programs
96
8 Using the Utilities
98
About
99
Control
100
Remote
100
Config
101
Set Date/Time
101
Bay Configuration
102
Bay Identification
102
Comm Config
102
Service
102
Network
103
Update Soft> & <Network Config
103
Gradient Calc
104
User Name
101
Transferring Program Files
104
10 Troubleshooting
110
Sources of Problems
111
System Problems
111
Error Messages
112
Problems in Power-Up
117
Problems with System Performance
118
Problems Related to Protocols
119
Problems with an Alpha Unit
119
Problems Due to Environmental Conditions, Setup, and Maintenance
122
Appendix A: Warranties
124
Chapter 10 : Troubleshooting
124
Chapter 8 : Using the Utilities
124
Chapter 9 : Maintenance
124
Appendix B: End-User and License Agreement
128
Appendix C: Factory-Installed Protocols
132
Index
134
Declaration of Conformity
146
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