Cleaning Radioactive or Biohazardous Materials out of the
Block
When cleaning instruments that have been running radioactive or biohazardous reac-
tions, consult your institution's radiation safety officer or biosafety officer regarding
cleaning methods, monitoring, and disposing of contaminated materials.
Changing the Fuses
The circuits in the DNA Engine cycler are protected by two fuses (6.3A fast-acting,
5x20mm). When a fuse blows, the DNA Engine cycler immediately shuts down and
cannot be turned back on. The machine records the event as a power loss, so if a
protocol is running when a fuse blows, the machine will resume the run when the fuse
is replaced and power restored (see "Resuming a Protocol after a Power Outage,"
chapter 5).
Warning: The DNA Engine cycler incorporates neutral fusing, which means
1. Disconnect the power cord from the back of the instrument. Move the power
switch to the "0" (off) position.
2. Insert one corner of a small flat-head screwdriver just under the fuse plug (fig. 10-1A),
and gently pry the plug loose. Pull the plug straight out as far as it will go, then push
it downward to expose the fuses (fig. 10-2B).
3. Remove both fuses and replace them with new ones (it is impossible to visually
determine which fuse is blown). You may also test the fuses with an ohmmeter to
determine which is defective and replace just that one.
4. Gently press the fuse cover back in place, and reconnect the power cord.
Figure 10-1
A, Removing the fuse plug.
A
that live power may still be available inside the unit even when a
fuse has blown or been removed. Never open the DNA Engine
base. You could receive a serious electrical shock. Opening the
base will also void your warranty.
Fuse plug
Screwdriver
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
B, Location of the fuses in the opened plug.
B
Fuse
Maintenance
Fuse
Fuse plug
10-3
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