BIO RAD PTC-200 Operation Manual page 94

Dna engine thermal cycler
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Error Message
Calc Control, Probe
Mode Invalid
Gradient Fault, Service
Alpha soon.
Gradient Program,
Invalid Alpha Type.
Heated Lid Fault,
Program Terminated.
HS Overheated,
Program Terminated
HS Overheating,
Check Air Flow
HS/PS Sensor Fault,
Program Terminated.
Cause and Result
Runtime error indicating a gradient
step is being programmed under
Probe Control mode.
Displayed at end of run. Indicates
gradient was not achieved within
20 sec of block reaching target.
Runtime error indicating gradient is
being programmed for an Alpha
module other than a 96V.
Lid sensor failed during preheat,
so Alpha unit has been shut down.
Machines are not getting enough
air, or air being taken in is warmer
than 31°C.
Machine is running a protocol con-
sisting of many cycles of only a few
seconds each. Alpha unit heat sink
does not have time to dissipate
heat generated by rapid cycling.
Eventually its maximum allowable
temperature is exceeded, and
Alpha unit is shut down.
Sensor malfunction has allowed
base to heat block over its max-
imum allowable temperature
(107.5°C), triggering automatic
Alpha unit shutdown.
See causes for "HS Overheated,
Program Terminated."
Heat sink sensor in Alpha unit and
power supply sensor in base are
not working, so Alpha unit has
been shut down.
Tech Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Troubleshooting
Action
Change to Calc control mode.
Alpha unit and / or base needs
servicing. Contact Bio-Rad or your
local distributor.
Use 96V Alpha nodule.
Heated lid or base needs servicing
Contact Bio-Rad or your local dis-
tributor.
Make sure machine gets enough
air and that temperature of air
being taken in is 31°C or cooler
(see p. 3-3). Correct air supply
problems and run protocol again.
If error message persists, base
may need servicing. Contact
Bio-Rad or your local distributor.
Contact Bio-Rad or your local dis-
tributor to discuss protocol.
Alpha unit and / or base needs
servicing. Contact Bio-Rad or
your local distributor.
See actions for "HS Overheated,
Program Terminated."
Alpha unit or base needs servicing.
Contact Bio-Rad or your local dis-
tributor.
11-3

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